MCCLATCHY J K, ROSENBLUM E D
J Bacteriol. 1963 Dec;86(6):1211-5. doi: 10.1128/jb.86.6.1211-1215.1963.
McClatchy, J. K. (The University of Texas, Dallas), and E. D. Rosenblum. Induction of lactose utilization in Staphylococcus aureus. J. Bacteriol. 86:1211-1215. 1963.-Adaptation to the utilization of lactose by Staphylococcus aureus has been compared with that by Escherichia coli. Lactose and galactose were found to be efficient inducers of the beta-galactosidase and the postulated galactoside-permease of S. aureus; the thiogalactosides, active as gratuitous inducers for E. coli, were inactive and inhibited induction of staphylococci by lactose and galactose. Mutants lacking beta-galactosidase or galactoside-permease, as well as a constitutive mutant for lactose utilization, were isolated. Like that in E. coli, the genetic system of staphylococcus seems to consist of two structural genes for the synthesis of the two enzymes and at least one regulatory gene simultaneously controlling the expression of the structural genes. The mutants were grouped by cross-transduction studies in three loci corresponding to the three phenotypes. Mutants of the pleiotropic locus were also isolated.
麦克拉奇，J.K.（得克萨斯大学达拉斯分校）和E.D.罗森布卢姆。金黄色葡萄球菌乳糖利用的诱导。《细菌学杂志》86:1211 - 1215。1963年。——已将金黄色葡萄球菌对乳糖利用的适应性与大肠杆菌的进行了比较。发现乳糖和半乳糖是金黄色葡萄球菌β - 半乳糖苷酶和假定的半乳糖苷通透酶的有效诱导剂；硫代半乳糖苷对大肠杆菌起 gratuitous诱导剂的作用，但对金黄色葡萄球菌无活性，且抑制乳糖和半乳糖对葡萄球菌的诱导。分离出了缺乏β - 半乳糖苷酶或半乳糖苷通透酶的突变体，以及乳糖利用的组成型突变体。与大肠杆菌类似，葡萄球菌的遗传系统似乎由两个用于合成两种酶的结构基因和至少一个同时控制结构基因表达的调节基因组成。通过交叉转导研究将突变体分为对应三种表型的三个位点。还分离出了多效性位点的突变体。
