Ruiz J, Núñez M L, Lorente I, Pérez J, Simarro E, Gómez J
Laboratory of Microbiology, Hospital Universitario Virgen de la Arrixaca, Murcia, Spain.
Eur J Clin Microbiol Infect Dis. 1996 Dec;15(12):922-6. doi: 10.1007/BF01690509.
The relative performance of five plating media [Rambach agar; salmonella-shigella (SS) agar, novobiocin-brilliant green-glycerol-lactose (NBGL), modified semisolid Rappaport-Vassiliadis medium (MSRV), and Salmonella Detection and Identification-2 (SM2)] and selenite broth (SB) subcultured in SS agar in the recovery of Salmonella spp. from 500 human stool specimens was evaluated. On Rambach agar and SS agar, the C8-esterase test was also used for selection of suspicious colonies. Eighty-one samples were positive for salmonellae on at least one of the six media. Sensitivities and specificities of MSRV, SB, NBGL, SS, Rambach agar, and SM2 were 95.1 and 98.1%, 87.6 and 99.8%, 79 and 91.9%, 69.1 and 99.3%, 56.8 and 96.9%, and 54.3 and 92.4%, respectively. There were statistically significant differences between MSRV and NBGL, Rambach agar, SS agar, and SM2 (p < 0.005), between SB and SS agar, Rambach agar, and SM2 (p < 0.05), and between NBGL and SM2 and Rambach agar (p < 0.005). The greatest number of isolates was recovered with MSRV, whose performance surpassed that of enrichment in selenite broth, probably because the subcultures were not repeated on MSRV. This hypothesis is now under investigation. The specificity of each of the five solid media was greater than 90%.
评估了五种平板培养基[兰巴赫琼脂；沙门氏菌-志贺氏菌（SS）琼脂、新生霉素-亮绿-甘油-乳糖（NBGL）、改良半固体拉帕波特-瓦西里亚迪斯培养基（MSRV）和沙门氏菌检测与鉴定-2（SM2）]以及在SS琼脂中传代培养的亚硒酸盐肉汤（SB）从500份人类粪便标本中分离沙门氏菌属的相对性能。在兰巴赫琼脂和SS琼脂上，C8酯酶试验也用于筛选可疑菌落。81份样本在六种培养基中的至少一种上沙门氏菌检测呈阳性。MSRV、SB、NBGL、SS、兰巴赫琼脂和SM2的敏感性和特异性分别为95.1%和98.1%、87.6%和99.8%、79%和91.9%、69.1%和99.3%、56.8%和96.9%、54.3%和92.4%。MSRV与NBGL、兰巴赫琼脂、SS琼脂和SM2之间存在统计学显著差异（p<0.005），SB与SS琼脂、兰巴赫琼脂和SM2之间存在统计学显著差异（p<0.05），NBGL与SM2和兰巴赫琼脂之间存在统计学显著差异（p<0.005）。MSRV分离出的菌株数量最多，其性能超过了亚硒酸盐肉汤增菌法，可能是因为MSRV上没有重复传代培养。这一假设目前正在研究中。五种固体培养基各自的特异性均大于90%。
