Ruiz J, Núñez M L, Díaz J, Sempere M A, Gómez J, Usera M A
Department of Microbiology, H. Virgen de la Arrixaca, Murcia, Spain.
J Appl Bacteriol. 1996 Nov;81(5):571-4. doi: 10.1111/j.1365-2672.1996.tb03549.x.
We studied the capacity of 10 selective media (Rambach agar, RB; salmonella-shigella agar, SS; SM-ID medium, SM; Hektoen enteric agar, HE; modified semisolid Rappaport-Vassiliadis agar, MSRV; bismuth sulphite agar, BS; MacConkey agar, MC; brilliant green agar, BG; novobiocin-brilliant green-glucose agar, NBG; and novobiocin-brilliant green-glycerol-lactose agar, NBGL), and the C8-esterase test (MUCAP test, Biolife, Italy) to detect the growth of 14 strains of lactose-positive Salmonella (12 Salm. virchow and two Salm. montevideo) and 16 Salm. arizonae. Suspensions of pure strain were plated on the aforementioned media and on Mueller-Hinton, used as a control, with inocula of 3 x 10(2) cfu ml-1. The performance of BS was excellent, determining the 30 strains as typical Salmonella colonies (H2S+). On NBG, 27 strains were detected. On MSRV, only some strains grew and only one produced swarming. On the other media, the two Salm. montevideo and the 12 Salm. virchow strains produced coliform colonies. Some of these latter were inhibited on BG and NBGL. The 16 Salm. arizonae strains produced typical colonies on all the media, except on RB, SM and MSRV. On NBGL, two strains did not produce H2S. The C8-esterase test was only successful with Salm. montevideo and Salm. virchow on NBG and RB (with a few exceptions on the latter). However, with Salm. arizonae the test was positive on SS, MC, HE, BG and NBG. In summary, BS was the best medium of those used (all the 30 strains were isolated), followed by NBG (27 isolates).
我们研究了10种选择性培养基（兰巴赫琼脂，RB；沙门氏菌-志贺氏菌琼脂，SS；SM-ID培养基，SM；赫克托恩肠道琼脂，HE；改良半固体拉帕波特-瓦西里亚迪斯琼脂，MSRV；亚硫酸铋琼脂，BS；麦康凯琼脂，MC；亮绿琼脂，BG；新生霉素-亮绿-葡萄糖琼脂，NBG；以及新生霉素-亮绿-甘油-乳糖琼脂，NBGL）和C8酯酶试验（MUCAP试验，意大利生物生命公司）检测14株乳糖阳性沙门氏菌（12株猪霍乱沙门氏菌和2株蒙得维的亚沙门氏菌）和16株亚利桑那沙门氏菌生长的能力。将纯菌株悬液接种于上述培养基以及用作对照的穆勒-欣顿培养基上，接种量为3×10² cfu/ml。BS的性能极佳，能将30株菌鉴定为典型沙门氏菌菌落（H2S阳性）。在NBG上，检测到27株菌。在MSRV上，只有部分菌株生长，且只有一株产生蔓延生长。在其他培养基上，2株蒙得维的亚沙门氏菌和12株猪霍乱沙门氏菌产生大肠菌群菌落。其中一些在BG和NBGL上受到抑制。16株亚利桑那沙门氏菌在所有培养基上均产生典型菌落，但在RB、SM和MSRV上除外。在NBGL上，有2株菌不产生H2S。C8酯酶试验仅在NBG和RB上对蒙得维的亚沙门氏菌和猪霍乱沙门氏菌成功（后者有少数例外）。然而，对于亚利桑那沙门氏菌，该试验在SS、MC、HE、BG和NBG上呈阳性。总之，在所使用的培养基中，BS是最佳培养基（分离出了所有30株菌），其次是NBG（分离出27株菌）。
