产气克雷伯菌谷氨酰胺合成酶：腺苷酸化系统中突变体的遗传和生理特性
Glutamine synthetase of Klebsiella aerogenes: genetic and physiological properties of mutants in the adenylylation system.
作者信息
Janssen K A, Magasanik B
出版信息
J Bacteriol. 1977 Feb;129(2):993-1000. doi: 10.1128/jb.129.2.993-1000.1977.
Abstract
Mutations resulting in defects in the adenylylation system of glutamine synthetase (GS) affect the expression of glnA, the structural gene for GS. Mutants with lesions in glnB are glutamine auxotrophs and contain repressed levels of highly adenylylated GS. Glutamine-independent revertants of the glnB3 mutant have acquired an additional mutation at the glnE site. The glnE54 mutant is incapable of adenylylating GS and produces high levels of enzyme, even when ammonia is present in the growth medium. The fact that mutations in glnB and glnE simultaneously disturb both the normal adenylylation and repression patterns of GS in Klebsiella aerogenes indicates that the adenylylation system, or adenylylation state, of GS is critical for the regulation of synthesis of GS.
摘要
导致谷氨酰胺合成酶（GS）腺苷酸化系统缺陷的突变会影响GS结构基因glnA的表达。glnB有损伤的突变体是谷氨酰胺营养缺陷型，且含有高度腺苷酸化的GS的受抑制水平。glnB3突变体的谷氨酰胺非依赖回复突变体在glnE位点获得了额外的突变。glnE54突变体无法对GS进行腺苷酸化，即使生长培养基中存在氨，也会产生高水平的酶。glnB和glnE中的突变同时干扰产气克雷伯菌中GS的正常腺苷酸化和抑制模式，这一事实表明GS的腺苷酸化系统或腺苷酸化状态对GS合成的调节至关重要。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Genetic and metabolic control of enzymes responsible for histidine degradation in Salmonella typhimurium. 4-imidazolone-5-propionate amidohydrolase and N-formimino-L-glutamate formiminohydrolase.鼠伤寒沙门氏菌中负责组氨酸降解的酶的遗传和代谢调控。4-咪唑酮-5-丙酸酰胺水解酶和N-甲酰亚氨基-L-谷氨酸甲酰亚氨基水解酶。
J Biol Chem. 1971 May 25;246(10):3320-9.
3
Multiple molecular forms of glutamine synthetase produced by enzyme catalyzed adenylation and deadenylylation reactions.通过酶催化的腺苷化和去腺苷化反应产生的谷氨酰胺合成酶的多种分子形式。
Adv Enzyme Regul. 1970;8:99-118. doi: 10.1016/0065-2571(70)90011-7.
4
Direct selection for P1-sensitive mutants of enteric bacteria.对肠道细菌的P1敏感突变体进行直接筛选。
J Bacteriol. 1974 Jun;118(3):810-4. doi: 10.1128/jb.118.3.810-814.1974.
5
Escherichia coli K-12 F-prime factors, old and new.大肠杆菌K-12 F-prime因子，新旧情况
Bacteriol Rev. 1972 Dec;36(4):587-607. doi: 10.1128/br.36.4.587-607.1972.
6
Biological function of the ammonia-induced inactivation of glutamine synthetase in Escherichia coli.氨诱导大肠杆菌谷氨酰胺合成酶失活的生物学功能。
Eur J Biochem. 1972 Mar 15;26(1):68-72. doi: 10.1111/j.1432-1033.1972.tb01740.x.
7
Modulation of glutamine synthetase adenylylation and deadenylylation is mediated by metabolic transformation of the P II -regulatory protein.谷氨酰胺合成酶腺苷酸化和去腺苷酸化的调节由P II调节蛋白的代谢转化介导。
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Proc Natl Acad Sci U S A. 1974 Jan;71(1):225-9. doi: 10.1073/pnas.71.1.225.
9
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J Biol Chem. 1973 Sep 10;248(17):6122-8.
10
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