Foor F, Janssen K A, Magasanik B
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4844-8. doi: 10.1073/pnas.72.12.4844.
We have examined three mutants of Klebsiella aerogenes whose genetic lesions (glnB, glnD, and glnE) are in loci unlinked to the structural gene for glutamine sythetase (glnA) and in which the control of both the level and state of adenylylation of glutamine synthetase is altered. Each mutation alters a different component of the adenylylation system of glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2]. Inability of the cell to deadenylylate glutamine synthetase (glnB and glnD) greatly decreases its production, while inability to adenylylate glutamine sythetase (glnE) results in its constitutively high production. These results together with our previous results indicate that adenylylated glutamine synthetase inhibits the transcription of glnA.
我们已经研究了产气克雷伯菌的三个突变体，其基因损伤（glnB、glnD和glnE）位于与谷氨酰胺合成酶结构基因（glnA）不连锁的位点，并且谷氨酰胺合成酶的腺苷酸化水平和状态的控制在这些突变体中发生了改变。每个突变改变了谷氨酰胺合成酶腺苷酸化系统的不同组分[L-谷氨酸：氨连接酶（ADP形成），EC 6.3.1.2]。细胞无法使谷氨酰胺合成酶去腺苷酸化（glnB和glnD）会大大降低其产量，而无法使谷氨酰胺合成酶腺苷酸化（glnE）则导致其组成型高产量。这些结果与我们之前的结果共同表明，腺苷酸化的谷氨酰胺合成酶抑制glnA的转录。
