Röcken C, Linke R P, Saeger W
Department of Pathology, Marienkrankenhaus Hamburg, Federal Republic of Germany.
Virchows Arch A Pathol Anat Histopathol. 1992;421(4):339-44. doi: 10.1007/BF01660981.
Immunoreactivity for islet amyloid polypeptide (IAPP) in the islets of Langerhans of non-insulin-dependent diabetic patients and non-diabetic patients of a non-selected post-mortem series was studied with a new polyclonal IAPP antibody. Out of 133 patients examined, 124 exhibited immunoreactivity for IAPP. Immunoreactivity was localized intra- and extracellularly and was limited to the islets of Langerhans. No extracellular immunoreactivity was observed in amyloid-negative cases. Co-localization of insulin and IAPP in the same islet-cells was verified by double staining with monoclonal insulin and polyclonal IAPP antibodies. Of 100 patients with non-insulin-dependent diabetes mellitus (NIDDM) and islet amyloid, 98 exhibited IAPP-positive deposits and 71 exhibited intracellular immunoreactivity. Evaluation of intracellular immunoreactivity and degree of islet amyloid deposition in cases of overt NIDDM revealed an inverse relationship, in that intracellular IAPP immunoreactivity were reduced in patients with developing islet amyloid deposition. Our data are consistent with the hypothesis of primary beta-cell dysfunction leading to amyloid formation, with subsequent disturbance of beta-cell homeostasis.
使用一种新的多克隆胰岛淀粉样多肽（IAPP）抗体，研究了非胰岛素依赖型糖尿病患者和非选择性尸检系列中的非糖尿病患者胰岛中IAPP的免疫反应性。在133例接受检查的患者中，124例表现出IAPP免疫反应性。免疫反应性定位于细胞内和细胞外，且仅限于胰岛。在淀粉样蛋白阴性病例中未观察到细胞外免疫反应性。通过单克隆胰岛素抗体和多克隆IAPP抗体双重染色，证实了胰岛素和IAPP在同一胰岛细胞中的共定位。在100例非胰岛素依赖型糖尿病（NIDDM）且有胰岛淀粉样变的患者中，98例表现出IAPP阳性沉积物，71例表现出细胞内免疫反应性。对显性NIDDM病例中的细胞内免疫反应性和胰岛淀粉样沉积程度的评估显示出一种负相关关系，即随着胰岛淀粉样沉积的发展，患者细胞内IAPP免疫反应性降低。我们的数据与原发性β细胞功能障碍导致淀粉样蛋白形成，随后扰乱β细胞内环境稳定的假说一致。
