Sebag J, Buckingham B, Charles M A, Reiser K
Doheny Eye Institute, University of Southern California, Los Angeles.
Arch Ophthalmol. 1992 Oct;110(10):1472-6. doi: 10.1001/archopht.1992.01080220134035.
Vitreous changes in diabetes can exacerbate proliferative diabetic retinopathy. These changes may be due to the effects of diabetes on vitreous collagen. Vitreous samples from 19 patients with proliferative diabetic retinopathy and 23 patients without diabetes were analyzed for collagen crosslinks, as well as for the early glycation products, glucitolyllysine and glucitolylhydroxylysine. Fluorometry was performed to measure advanced glycation end products. Vitreous collagen derived from diabetic patients was found to have significantly higher levels of the crosslink dihydroxylysinonorleucine (3.15 vs 1.24 mol/mol collagen, P<.01) than that of control subjects. Early glycation products were elevated in diabetic vitreous (1.65 vs 0.54 mol/mol collagen, P<.05). Levels of advanced glycation end products were 20 times higher in diabetic vitreous compared with the vitreous of controls. These diabetes-induced alterations of human vitreous may be of particular importance given the role of vitreous in proliferative diabetic retinopathy and vision loss.
糖尿病中的玻璃体变化可加剧增殖性糖尿病视网膜病变。这些变化可能是由于糖尿病对玻璃体胶原蛋白的影响。对19例增殖性糖尿病视网膜病变患者和23例非糖尿病患者的玻璃体样本进行了胶原蛋白交联分析，以及早期糖基化产物、葡糖基赖氨酸和葡糖基羟赖氨酸的分析。采用荧光法测定晚期糖基化终产物。发现糖尿病患者的玻璃体胶原蛋白中交联二羟基赖氨酰正亮氨酸的水平（3.15对1.24摩尔/摩尔胶原蛋白，P<0.01）显著高于对照组。糖尿病玻璃体中的早期糖基化产物升高（1.65对0.54摩尔/摩尔胶原蛋白，P<0.05）。糖尿病玻璃体中晚期糖基化终产物的水平比对照组玻璃体高20倍。鉴于玻璃体在增殖性糖尿病视网膜病变和视力丧失中的作用，这些由糖尿病引起的人类玻璃体改变可能尤为重要。
