Dejmek A, Strömberg C, Wikström B, Hjerpe A
Department of Pathology II, Karolinska Institute, Huddinge University Hospital, Sweden.
Anal Quant Cytol Histol. 1992 Jun;14(3):217-21.
Malignant mesotheliomas often raise a difficult diagnostic problem: once the diagnosis is made, the possibilities of predicting the biologic activity and prognosis of the tumor are limited. DNA ploidy patterns have been used as a prognostic instrument for other tumors, and this pattern was therefore studied in 37 cases of verified mesothelioma. The measurements were made on Feulgen-stained smears from pleural effusions using a Leica Miamed computer microscope. When a highly aneuploid tumor was defined as a condition with greater than 5% of the cells within defined intervals outside the ranges of the stem-line and the peaks representing polyploidization thereof, a near-diploid/polyploid pattern was obtained in 41% of the cases, while the remaining 59% were classified as highly aneuploid. The prognosis was significantly better among the near-diploid/polyploid cases. This estimate of the outcome could not be improved by using alternative algorithms for high-grade aneuploidy.
一旦做出诊断，预测肿瘤生物学活性和预后的可能性就很有限。DNA倍体模式已被用作其他肿瘤的预后指标，因此对37例经证实的间皮瘤病例进行了该模式的研究。使用徕卡Miamed计算机显微镜对经福尔根染色的胸腔积液涂片进行测量。当将高度非整倍体肿瘤定义为在干细胞系范围和代表其多倍体化的峰范围之外的特定区间内有超过5%的细胞的情况时，41%的病例呈现近二倍体/多倍体模式，而其余59%被归类为高度非整倍体。近二倍体/多倍体病例的预后明显更好。使用其他高级别非整倍体算法并不能改善对结果的这种估计。
