Holländer R, Wolf G, Mannheim W
Antonie Van Leeuwenhoek. 1977;43(2):177-85. doi: 10.1007/BF00395672.
In a comparative study the lipoquinones of some chemoorganotrophic, facultatively aerobic bacteria, and representative Acholeplasma, Mycoplasma, Spiroplasma, and Thermoplasma strains were investigated. The quinones were partly purified by preparative thin layer chromatography of lipid extracts, and characterized by their difference spectra (reduced minus oxidized) and Rf values. Respiring bacteria expectedly contained benzoquinones and/or naphthoquinones in micromolar concentrations whereas some aerotolerant, cytochrome-less, gram-positive bacteria were found to contain menaquinones in nanomolar concentrations, or even no quinones; only Streptococcus faecalis, an organism supposed to use a rudimentary, flavin-terminated respiratory chain system produced desmethyl menaquinone in amounts ranging between "high" and "low" quinone contents. Among the mycoplasmas investigated, only Thermoplasma acidophilum was found to be capable of synthesizing quinones (MK-7) in the micromolar order of magnitude indicating a respiratory electron transport system. The presence of energetically useful respiratory chain systems in Acholeplasma, Mycoplasma, and Spiroplasma is questioned since these organisms contain quinones (MK-4) in nanomolar concentrations, or no quinones, depending on the presence of exogeneous MK-6 in the growth medium. The possible metabolite role of menaquinones present in "low" amounts, as well as the role of NADH oxidase systems more or less tightly bound to the cytoplasmic membrane with the mycoplasmas deserves further investigation.
在一项比较研究中，对一些化能有机营养型兼性需氧细菌以及代表性的无胆甾原体、支原体、螺原体和热原体菌株的脂醌进行了研究。通过脂质提取物的制备型薄层色谱法对醌进行了部分纯化，并通过其差示光谱（还原态减去氧化态）和比移值进行了表征。需氧呼吸细菌预期含有微摩尔浓度的苯醌和/或萘醌，而一些耐氧、无细胞色素的革兰氏阳性细菌被发现含有纳摩尔浓度的甲基萘醌，甚至不含醌；只有粪肠球菌，一种被认为使用基本的、以黄素为末端的呼吸链系统的生物体，产生的去甲基甲基萘醌量在“高”和“低”醌含量之间。在所研究的支原体中，只有嗜热栖热菌被发现能够合成微摩尔数量级的醌（MK-7），这表明存在呼吸电子传递系统。无胆甾原体、支原体和螺原体中是否存在高效的呼吸链系统受到质疑，因为这些生物体根据生长培养基中是否存在外源MK-6，含有纳摩尔浓度的醌（MK-4）或不含醌。支原体中少量存在的甲基萘醌可能的代谢作用，以及与细胞质膜或多或少紧密结合的NADH氧化酶系统的作用，值得进一步研究。
