白喉毒素作用机制的研究。IV. 无细胞体系中毒素作用对辅因子（NAD）需求的特异性。
Studies on the mode of action of diphtheria toxin. IV. Specificity of the cofactor (NAD) requirement for toxin action in cell-free systems.
作者信息
Goor R S, Pappenheimer A M
出版信息
J Exp Med. 1967 Nov 1;126(5):913-21. doi: 10.1084/jem.126.5.913.
Abstract
The ability of a number of nucleotides related to NAD to replace NAD as cofactors for inhibition by diphtheria toxin of peptide bond formation has been examined. Neither NADH nor NADP are active. Of some 14 analogues closely related structurally to NAD that have been tested, only 3-thiocarboxamide pyridine-AD is as active as NAD itself. Replacement of the 3-carboxamide group on the pyridine ring by an acetyl group, or deamination of the purine ring, resulted in derivatives with reduced activity. The results were interpreted as suggesting that NAD and certain related nucleotides are capable of specific interaction with diphtheria toxin. Using the method of equilibrium dialysis, reversible binding of 1 mole of NAD per mole of toxin has been demonstrated. Toxoid does not interact with NAD.
摘要
已对一些与烟酰胺腺嘌呤二核苷酸（NAD）相关的核苷酸作为辅因子替代NAD以抑制白喉毒素对肽键形成的能力进行了研究。烟酰胺腺嘌呤二核苷酸磷酸（NADH）和烟酰胺腺嘌呤二核苷酸磷酸（NADP）均无活性。在已测试的约14种结构上与NAD密切相关的类似物中，只有3-硫代甲酰胺吡啶-AD与NAD本身活性相同。吡啶环上的3-甲酰胺基团被乙酰基取代，或嘌呤环脱氨基，会产生活性降低的衍生物。这些结果被解释为表明NAD和某些相关核苷酸能够与白喉毒素发生特异性相互作用。使用平衡透析法，已证明每摩尔毒素可与1摩尔NAD发生可逆结合。类毒素不与NAD相互作用。
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本文引用的文献
1
[AMINO ACID COMPOSITION OF PURIFIED DIPHTHERIA TOXIN].[纯化白喉毒素的氨基酸组成]
Bull Soc Chim Biol (Paris). 1965;47:261-6.
2
STUDIES ON THE MODE OF ACTION OF DIPHTHERIA TOXIN. II. EFFECT OF TOXIN ON AMINO ACID INCORPORATION IN CELL-FREE SYSTEMS.白喉毒素作用方式的研究。II. 毒素对无细胞体系中氨基酸掺入的影响。
J Exp Med. 1964 Dec 1;120(6):1019-39. doi: 10.1084/jem.120.6.1019.
3
Measurement of protein-binding phenomena by gel filtration.通过凝胶过滤法测定蛋白质结合现象。
Biochim Biophys Acta. 1962 Oct 8;63:530-2. doi: 10.1016/0006-3002(62)90124-5.
4
A streptococcal enzyme that acts specifically upon diphosphopyridine nucleotide: characterization of the enzyme and its separation from streptolysin O.一种对二磷酸吡啶核苷酸具有特异性作用的链球菌酶：该酶的特性及其与链球菌溶血素O的分离
J Exp Med. 1957 Jul 1;106(1):15-26. doi: 10.1084/jem.106.1.15.
5
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Biochem J. 1955 Nov;61(3):381-8. doi: 10.1042/bj0610381.
6
Kinetics of cytochrome b5 in rat liver microsomes.大鼠肝脏微粒体中细胞色素b5的动力学
J Biol Chem. 1954 Aug;209(2):945-51.
7
The interaction of aminoacyl transferase II and ribosomes.氨酰基转移酶II与核糖体的相互作用。
J Biol Chem. 1966 Apr 25;241(8):1698-704.
8
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J Mol Biol. 1967 Apr 14;25(1):83-98. doi: 10.1016/0022-2836(67)90280-x.
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