用于鉴定链球菌M蛋白抗体的微量滴定间接血凝试验
Microtiter indirect hemagglutination procedure for identification of streptococcal M-protein antibodies.
作者信息
Zimmerman R A, Mathews J, Wilson E
出版信息
Appl Microbiol. 1968 Nov;16(11):1640-5. doi: 10.1128/am.16.11.1640-1645.1968.
Abstract
A number of investigators have attempted to utilize the hemagglutination system for detection of streptococcal type-specific antibody in human sera. Cross-reactions have made the procedure unreliable without cumbersome and time-consuming manipulation of the test sera. A method is described in which a microtiter indirect hemagglutination technique, using sensitized sheep erythrocytes, is sensitive, specific, and reliable for titration of type-specific antibody after naturally acquired or induced streptococcal infection.
摘要
许多研究人员试图利用血凝系统检测人血清中的链球菌型特异性抗体。交叉反应使得该程序不可靠，除非对测试血清进行繁琐且耗时的处理。本文描述了一种方法，即使用致敏绵羊红细胞的微量滴定间接血凝技术，对于自然获得或诱导的链球菌感染后型特异性抗体的滴定而言，该技术灵敏、特异且可靠。
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本文引用的文献
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STREPTOCOCCAL M PROTEIN ANTIBODIES.链球菌M蛋白抗体
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Long chain formation by strains of group A streptococci in the presence of homologous antiserum: a type-specific reaction.A组链球菌菌株在同源抗血清存在下形成长链：一种型特异性反应。
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The indirect bactericidal test as a means of identifying antibody to the M antigen of Streptococcus pyogenes.间接杀菌试验作为鉴定抗化脓性链球菌M抗原抗体的一种方法。
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