Brunmark P, Persson P, Skarping G
Department of Occupational and Environmental Medicine, University Hospital, Lund Sweden.
J Chromatogr. 1992 Sep 2;579(2):350-4. doi: 10.1016/0378-4347(92)80403-d.
4,4'-Methylenedianiline was determined in human urine by micro liquid chromatography with ultraviolet detection. The combination of a thorough work-up and the high mass sensitivity of micro liquid chromatography gave the method very high sensitivity. Derivatization with pentafluoropropionic anhydride enhanced the resolution of the 4,4'-dimethylenedianiline peak. The detection limit, defined as blank plus three times the standard deviation of the blank, was 2 nmol/l of urine, for 10-microliters injection volumes. The detection limit, defined as three times the noise, was about ten times better. The within- and between-assay coefficients of variation were 4 and 6%, respectively, for samples containing 40 nmol/l. The method was applied for the monitoring of excreted 4,4'-methylenedianiline in urine, during epicutaneous skin hypersensitivity testing (patch testing).
采用带紫外检测的微液相色谱法测定人尿中的4,4'-亚甲基二苯胺。彻底的样品前处理与微液相色谱的高灵敏度相结合，使该方法具有很高的灵敏度。用五氟丙酸酐衍生化提高了4,4'-亚甲基二苯胺峰的分离度。对于10微升进样体积，定义为空白加三倍空白标准偏差的检测限为2纳摩尔/升尿液。定义为三倍噪声的检测限约好十倍。对于含有40纳摩尔/升的样品，批内和批间变异系数分别为4%和6%。该方法用于在皮肤超敏反应试验（斑贴试验）期间监测尿中排泄的4,4'-亚甲基二苯胺。
