Brand C U, Hunziker T, Braathen L R
Dermatological Clinic, University of Berne, Switzerland.
J Invest Dermatol. 1992 Nov;99(5):109S-110S. doi: 10.1111/1523-1747.ep12669997.
Immunologic processes in diseased human skin have been extensively investigated, but little is known about the effect of skin diseases on human afferent skin lymph. Starting in the papillary dermis, the skin lymphatics drain the adjacent tissue in a one-way flow toward the regional lymph nodes. The composition of the afferent lymph, therefore, reflects the immunologic inflammatory processes in the drained tissue. To obtain afferent lymph to investigate its content, we inserted a cannula, by means of microsurgery, into a superficial peripheral lymph vessel draining a defined skin area. By manipulating the drained skin area and subsequent examination of the lymph we established an in vivo system for investigating the kinetics of lymph changes during the course of skin reactions. In lymph derived from a mild sodium lauryl sulphate (SLS)--induced contact dermatitis we could demonstrate an increase of both flow and cells. In particular, the number of Langerhans cells (LC) increased enormously during the course of the skin reaction. It, therefore, seems that a large increase in the migration of LC from the skin to the regional lymph nodes is a major feature of SLS-induced contact dermatitis, suggesting that LC may play a major role in the irritant contact dermatitis reaction.
人类患病皮肤中的免疫过程已得到广泛研究，但皮肤疾病对人体传入性皮肤淋巴的影响却知之甚少。皮肤淋巴管始于乳头层真皮，以单向流动的方式将相邻组织引流至区域淋巴结。因此，传入淋巴的成分反映了引流组织中的免疫炎症过程。为获取传入淋巴以研究其成分，我们通过显微外科手术将一根套管插入引流特定皮肤区域的浅表外周淋巴管。通过操控引流的皮肤区域并随后对淋巴进行检测，我们建立了一个用于研究皮肤反应过程中淋巴变化动力学的体内系统。在源自轻度十二烷基硫酸钠（SLS）诱导的接触性皮炎的淋巴中，我们能够证明流量和细胞数量均增加。特别是，在皮肤反应过程中朗格汉斯细胞（LC）的数量大幅增加。因此，似乎LC从皮肤向区域淋巴结迁移的大幅增加是SLS诱导的接触性皮炎的一个主要特征，这表明LC可能在刺激性接触性皮炎反应中起主要作用。
