Brand C U, Hunziker T, Braathen L R
Dermatological Clinic, Inselspital, University of Berne, Switzerland.
Arch Dermatol Res. 1992;284(3):123-6. doi: 10.1007/BF00372702.
By means of microsurgery a peripheral subcutaneous lymph vessel draining a defined skin area was isolated and cannulated on the lower leg of six healthy volunteers. Lymph was collected over a period of 8 days. During the first 2 days baseline values for lymph flow and output of cells were established. A contact dermatitis was then induced in the drained skin area by the application of 10% sodium lauryl sulphate. All six probands developed a mild to moderate irritant contact dermatitis. Lymph flow as well as output of cells increased with the intensity of the skin reaction. Subsequent local treatment with clobetasol propionate decreased the cell output, but the lymph flow increased further. Neither lymph flow nor output of cells returned to the initial baseline values at the end of the study, when the clinical signs of contact dermatitis had completely disappeared. During the experiment significant individual variations were found, with means ranging from 0.10 to 0.48 ml/h for lymph flow and from 8700 to 174000/h for cells, which probably depended mainly on the different topographies and calibres of the cannulated lymph vessels.
通过显微外科手术，在6名健康志愿者的小腿上分离并插管引流特定皮肤区域的外周皮下淋巴管。在8天的时间里收集淋巴液。在最初的2天里确定了淋巴液流量和细胞输出的基线值。然后通过涂抹10%的十二烷基硫酸钠在引流的皮肤区域诱发接触性皮炎。所有6名受试者均出现了轻度至中度的刺激性接触性皮炎。淋巴液流量以及细胞输出随着皮肤反应的强度而增加。随后用丙酸氯倍他索进行局部治疗降低了细胞输出，但淋巴液流量进一步增加。在研究结束时，当接触性皮炎的临床症状完全消失时，淋巴液流量和细胞输出均未恢复到初始基线值。在实验过程中发现了显著的个体差异，淋巴液流量的平均值在0.10至0.48毫升/小时之间，细胞的平均值在8700至174000/小时之间，这可能主要取决于插管淋巴管的不同位置和管径。
