Kohama T
Department of Urology, Akita University School of Medicine.
Nihon Hinyokika Gakkai Zasshi. 1992 Sep;83(9):1469-77. doi: 10.5980/jpnjurol1989.83.1469.
Input neuronal structures to the nucleus locus subcoeruleus (LSC) and the nucleus reticularis pontis oralis (PoO) were investigated by the horseradish peroxidase (HRP) study in cats. Under halothane anesthesia, a double barreled electrode was inserted into the LSC where electrical stimulation increased bladder capacity and the external urethral sphincter muscle activity, and into the PoO where chemical stimulation with carbachol increased bladder capacity and decreased the external urethral sphincter muscle activity. After identification of these regions, the HRP was ionphoretically injected into the LSC or PoO. By injecting the HRP into the LSC, retrogradely HRP labeled cells were located broadly in the frontal, rectal, orbitalis, rostral cingulate, internal aspect of posterior sigmoidal and anterior sylvian gyli, nucleus corticomedialis of amygdala, lateral area of the hypothalamus, paraventricular hypothalamic nucleus, substantia nigra, periaqueductal gray, reticular formation of the mesencephalon, pons and medulla, cerebellar nuclei and intermediate gray of the spinal cord. By injecting the HRP into the PoO, retrogradely HRP labeled cells were located broadly in the frontal, rectal, orbitalis, internal aspect of the posterior sigmoidal and anterior sylvian gyli, lateral area of the hypothalamus, paraventricular hypothalamic nucleus, periaqueductal gray, reticular formation of the mesencephalon, pons and medulla, cerebellar nuclei and intermediate gray of the spinal cord. These areas where HRP labeled cells were located mostly corresponded to the areas where electrical stimulation evoked either bladder relaxation or contraction in the previous reports. The LSC and the PoO seem to perform important roles in the neuronal mechanism for urine storage, receiving the inputs which facilitate or inhibit micturition from the extended areas between the cerebral cortex and the sacral spinal cord.
通过在猫身上进行的辣根过氧化物酶（HRP）研究，对投射到蓝斑下核（LSC）和脑桥嘴侧网状核（PoO）的输入神经元结构进行了研究。在氟烷麻醉下，将双管电极插入LSC，电刺激可增加膀胱容量和尿道外括约肌活动；还插入PoO，用卡巴胆碱进行化学刺激可增加膀胱容量并降低尿道外括约肌活动。在确定这些区域后，将HRP离子导入LSC或PoO。通过将HRP注入LSC，逆行标记的HRP细胞广泛分布在额叶、直肠叶、眶回、扣带回前部、乙状窦后回和颞上回内侧面、杏仁核皮质内侧核、下丘脑外侧区、下丘脑室旁核、黑质、导水管周围灰质、中脑、脑桥和延髓的网状结构、小脑核以及脊髓中间灰质。通过将HRP注入PoO，逆行标记的HRP细胞广泛分布在额叶、直肠叶、眶回、乙状窦后回和颞上回内侧面、下丘脑外侧区、下丘脑室旁核、导水管周围灰质、中脑、脑桥和延髓的网状结构、小脑核以及脊髓中间灰质。这些HRP标记细胞所在的区域大多与先前报道中电刺激诱发膀胱舒张或收缩的区域相对应。LSC和PoO似乎在尿液储存的神经元机制中发挥重要作用，接收来自大脑皮层和骶髓之间广泛区域的促进或抑制排尿的输入。
