Yamada S, Cao J, Sumita O, Kurasawa K, Kurata H, Oh K, Matsuoka H
Research and Development Division, Bio-Giken Inc., Tokyo, Japan.
Mycopathologia. 1992 May;118(2):65-9. doi: 10.1007/BF00442533.
A system for the evaluation of antifungal activity of volatile compounds has been developed that is based on dynamic growth of a single hypha. The newly developed system is composed of a reaction vessel under a microscope, automatic stage, charge coupled device (CCD) camera, TV monitor, video tape recorder (VTR), and a microcomputer. A fungus was inoculated in the reaction vessel containing agar medium and then was treated with an antifungal reagent in the gas phase either in batch or flow reaction manner. The apex of a growing hypha displayed on a TV monitor was followed automatically. From the ratio of the growth rate under exposure of a reagent (UEXPO) to the growth rate before the exposure (UPRE), the antifungal activity was expressed quantitatively.
已开发出一种基于单个菌丝动态生长来评估挥发性化合物抗真菌活性的系统。新开发的系统由显微镜下的反应容器、自动载物台、电荷耦合器件（CCD）相机、电视监视器、录像机（VTR）和微型计算机组成。将一种真菌接种到含有琼脂培养基的反应容器中，然后以分批或流动反应方式用气相抗真菌试剂进行处理。电视监视器上显示的生长菌丝顶端会被自动跟踪。根据试剂暴露下的生长速率（UEXPO）与暴露前的生长速率（UPRE）之比，对抗真菌活性进行定量表达。
