Oh K, Matsuoka H, Sumita O, Takatori K, Kurata H
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
Appl Microbiol Biotechnol. 1993 Mar;38(6):790-4. doi: 10.1007/BF00167146.
An automatic analysing system was developed and employed for the evaluation of antifungal activity of volatile compounds in the gas phase. Aspergillus niger was inoculated on agar medium in the reaction vessel. The reaction vessel was incubated at 28 degrees C for 24 h and then a volatile compound was introduced into the vessel either in a batch or flow manner. The antifungal activity of the respective compounds estimated in situ was expressed by the dynamic response parameters of a single hypha. All volatiles tested in the present system inhibited hyphal growth, except linalyl acetate: Limone and geraniol were the most inhibitory. In contrast, linalyl acetate promoted hyphal growth. By definition of the parameters, the fungicidal and fungistatic effects could be distinguished.
开发并采用了一种自动分析系统来评估气相中挥发性化合物的抗真菌活性。将黑曲霉接种在反应容器的琼脂培养基上。将反应容器在28℃下孵育24小时，然后以分批或流动方式将挥发性化合物引入容器中。原位评估的各化合物的抗真菌活性由单个菌丝的动态响应参数表示。在本系统中测试的所有挥发物均抑制菌丝生长，但乙酸芳樟酯除外：柠檬烯和香叶醇的抑制作用最强。相反，乙酸芳樟酯促进菌丝生长。根据参数的定义，可以区分杀菌和抑菌作用。
