Department of Geology and Geophysics, University of Wisconsin, Madison, WI 53706, USA.
Appl Environ Microbiol. 2009 Nov;75(21):6937-40. doi: 10.1128/AEM.01742-09. Epub 2009 Sep 11.
16S rRNA gene libraries from the lithoautotrophic Fe(II)-oxidizing, nitrate-reducing enrichment culture described by Straub et al. (K. L. Straub, M. Benz, B. Schink, and F. Widdel, Appl. Environ. Microbiol. 62:1458-1460, 1996) were dominated by a phylotype related (95% 16S rRNA gene homology) to the autotrophic Fe(II) oxidizer Sideroxydans lithotrophicus. The libraries also contained phylotypes related to known heterotrophic nitrate reducers Comamonas badia, Parvibaculum lavamentivorans, and Rhodanobacter thiooxidans. The three heterotrophs were isolated and found to be capable of only partial (12 to 24%) Fe(II) oxidation, suggesting that the Sideroxydans species has primary responsibility for Fe(II) oxidation in the enrichment culture.
16S rRNA 基因文库来自 Straub 等人描述的自养 Fe(II)氧化、硝酸盐还原富集培养物（K. L. Straub、M. Benz、B. Schink 和 F. Widdel，Appl. Environ. Microbiol. 62:1458-1460, 1996），主要由与自养 Fe(II)氧化剂 Sideroxydans lithotrophicus 相关的（95% 16S rRNA 基因同源性）的类群组成。文库还包含与已知异养硝酸盐还原剂 Comamonas badia、Parvibaculum lavamentivorans 和 Rhodanobacter thiooxidans 相关的类群。这三种异养菌被分离出来，并发现它们只能部分（12 到 24%）氧化 Fe(II)，这表明 Sideroxydans 物种是富集培养物中 Fe(II)氧化的主要责任菌。
