Rowlands R T, Turner G
Mol Gen Genet. 1977 Sep 9;154(3):311-8. doi: 10.1007/BF00571288.
The extranuclear mitochondrial oligomycin-resistant mutation of Aspergillus nidulans, (oliA1), was transferred asexually into four nuclear oligomycin-resistant strains of different phenotypes. In all four cases, the possession of the nuclear plus extranuclear mutation led to an increase in the in vivo level of oligomycin resistance. In two cases, the altered cytochrome spectrum and impaired growth ability determined by (oliA1) were suppressed by the nuclear mutations. In the third case, the in vitro oligomycin resistance of the double mutant ATPase was dramatically increased above that of either of the component single mutant strains, indicating a synergystic interaction between the nuclear and extranuclear gene products. In the fourth case, the double mutant became cold-sensitive. A new extranuclear mitochondrial oligomycin-resistant mutation (oliB332) is described. This mutant is phenotypically similar to, though not identical with, (oliA1) but is separable by recombination. A range of nuclear oligomycin-resistant mutants have been mapped. Despite presenting five distinctly different phenotypes, they all map at the same locus.
构巢曲霉的核外线粒体抗寡霉素突变（oliA1）通过无性方式转移到四个具有不同表型的核抗寡霉素菌株中。在所有四种情况下，同时拥有核突变和核外突变会导致体内抗寡霉素水平升高。在两种情况下，由（oliA1）决定的细胞色素光谱改变和生长能力受损被核突变所抑制。在第三种情况下，双突变体ATP酶的体外抗寡霉素能力比任何一个单突变体菌株都显著增强，表明核基因产物与核外基因产物之间存在协同相互作用。在第四种情况下，双突变体对温度敏感。本文描述了一种新的核外线粒体抗寡霉素突变（oliB332）。该突变体在表型上与（oliA1）相似，但不完全相同，且可通过重组分离。已经绘制了一系列核抗寡霉素突变体的图谱。尽管呈现出五种明显不同的表型，但它们都位于同一基因座上。
