Ward M, Wilson L J, Carmona C L, Turner G
Genencor, Incorporated, South San Francisco, CA 94080.
Curr Genet. 1988 Jul;14(1):37-42. doi: 10.1007/BF00405851.
The oliC3 gene of Aspergillus niger has been isolated and sequenced. This gene encodes an oligomycin-resistant variant of the mitochondrial ATP synthase subunit 9. In transformation experiments the gene can serve as a semi-dominant selectable marker for A. niger. It was possible to recognize transformants in which oliC3 had integrated at the homologous oliC locus, as opposed to elsewhere in the genome, by observation of phenotypes on medium containing oligomycin. DNA sequencing has allowed comparison of the deduced amino acid sequence with subunit 9 proteins from other species and comparison of 5' untranslated sequences with those from other fungi.
黑曲霉的oliC3基因已被分离和测序。该基因编码线粒体ATP合酶亚基9的一种抗寡霉素变体。在转化实验中，该基因可作为黑曲霉的半显性选择标记。通过观察含有寡霉素的培养基上的表型，能够识别oliC3在同源oliC位点而非基因组其他位置整合的转化体。DNA测序使得推导的氨基酸序列能够与其他物种的亚基9蛋白进行比较，以及5'非翻译序列能够与其他真菌的序列进行比较。
