Das Gupta S, Ghosh D K, Majumder H K
Leishmania Group, Indian Institute of Chemical Biology, Calcutta.
Parasitology. 1991 Apr;102 Pt 2:187-91. doi: 10.1017/s0031182000062478.
DNA-DNA hybridization techniques have been found to be very useful in the classification and identification of Leishmania parasites. We report here the cloning of a mini-circle from Leishmania strain UR6 in a plasmid and subcloning of the mini-circle fragments in M13 mp9. Clone MLURk32, containing a 560 bp fragment of mini-circle, has been found to have unique specificity. Application of this specific probe in identifying different Leishmania isolates reveals that the probe reacted only with strains of post-kala-azar dermal leishmaniasis but not with strains or isolates of visceral leishmaniasis.
已发现DNA-DNA杂交技术在利什曼原虫寄生虫的分类和鉴定中非常有用。我们在此报告从利什曼原虫UR6菌株中克隆出一个小环并将其克隆到质粒中，然后将小环片段亚克隆到M13 mp9中。已发现含有560 bp小环片段的克隆MLURk32具有独特的特异性。将这种特异性探针应用于鉴定不同的利什曼原虫分离株，结果显示该探针仅与黑热病后皮肤利什曼病的菌株反应，而不与内脏利什曼病的菌株或分离株反应。
