Ramos P L, Guevara-González R G, Peral R, Ascencio-Ibañez J T, Polston J E, Argüello-Astorga G R, Vega-Arreguín J C, Rivera-Bustamante R F
División de Plantas, Centro de Ingeniería Genética y Biotecnología, La Habana, Cuba.
Arch Virol. 2003 Sep;148(9):1697-712. doi: 10.1007/s00705-003-0136-3.
Over the last decade, the tomato production in Cuba has been affected by new whitefly-associated diseases. In addition to the well-documented presence of Tomato yellow leaf curl virus (TYLCV) along the island, the occurrence of bipartite begomoviruses has also been reported. One of them, tentatively named Tomato mottle Taino virus (ToMoTV), has now been cloned and characterized at the molecular level. Its genomic organization is similar to other bipartite geminiviruses. Phylogenetic analyses placed ToMoTV in a subcluster with other geminiviruses isolated in the Caribbean Basin: Tomato mottle virus (ToMoV), Bean dwarf mosaic virus, Abutilon mosaic virus, Sida golden mosaic virus and Potato yellow mosaic virus (PYMV). Biolistic inoculation of tobacco and tomato plants with cloned viral DNA showed that ToMoTV pseudorecombines with PYMV-GP as predicted by the identity of their iterative elements, whereas it does not show the same ability with ToMoV, even when their replication-associated proteins (Rep and REn) show the highest percentage of similarity. A comparative analysis of Rep proteins from begomoviruses that are able to produce viable reassortants suggests that some key elements for virus replication specificity are located in the first ten amino acids of this protein.
在过去十年中，古巴的番茄生产受到了与粉虱相关的新病害的影响。除了该岛已被充分记录的番茄黄化曲叶病毒（TYLCV）的存在外，双分体菜豆金色花叶病毒属病毒的出现也有报道。其中一种暂命名为番茄斑驳泰诺病毒（ToMoTV），现已在分子水平上进行了克隆和鉴定。其基因组结构与其他双分体双生病毒相似。系统发育分析将ToMoTV置于与在加勒比海盆地分离出的其他双生病毒的一个亚群中：番茄斑驳病毒（ToMoV）、菜豆矮化花叶病毒、苘麻花叶病毒、黄细心金色花叶病毒和马铃薯黄色花叶病毒（PYMV）。用克隆的病毒DNA对烟草和番茄植株进行基因枪接种表明，正如根据其重复元件的一致性所预测的那样，ToMoTV与PYMV-GP发生假重组，而即使ToMoTV与ToMoV的复制相关蛋白（Rep和REn）显示出最高百分比的相似性，它与ToMoV也不具有相同的假重组能力。对能够产生可行重配体的双生病毒的Rep蛋白进行的比较分析表明，病毒复制特异性的一些关键元件位于该蛋白的前十个氨基酸中。
