Saad Mabroka H, Sidkey Nagwa M, El-Fakharany Esmail M
Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El Arab, Alexandria, Egypt.
Botany & Microbiology Department, Faculty of Science, Al Azhar University (Girls Branch), Nasr City, Egypt.
Microb Cell Fact. 2024 Apr 22;23(1):117. doi: 10.1186/s12934-024-02383-4.
Several antiviral agents lost their efficacy due to their severe side effects and virus mutations. This study aimed to identify and optimize the conditions for exopolysaccharide (EPS) production from a newly isolated cyanobacterium, Acaryochloris Al-Azhar MNE ON864448.1, besides exploring its antiviral activity. The cyanobacterial EPS was purified through DEAE-52 cellulose column with a final yield of 83.75%. Different analysis instruments were applied for EPS identification, including Fourier-transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), and gas chromatographic-mass spectrometry (GC-MS). Plackett-Burman's design demonstrated that working volume (X1), EDTA (X2), inoculum size (X3), CaCl (X4), and NaCl (X5) are the most important variables influencing EPS production. Central composite design (CCD) exhibited maximum EPS yield (9.27 mg/mL) at a working volume of 300 mL in a 1 L volumetric flask, EDTA 0.002 g/L, inoculum size 7%, CaCl 0.046 g/L, and NaCl 20 g/L were applied. EPS showed potent antiviral activities at different stages of herpes simplex virus type-1 and 2 (HSV-1, HSV-2), adenovirus (ADV) and coxsackievirus (A16) infections. The highest half-maximal inhibitory concentration (IC) (6.477 µg/mL) was recorded during HSV-1 internalization mechanism, while the lowest IC (0.005669 µg/mL) was recorded during coxsackievirus neutralization mechanism.
几种抗病毒药物由于其严重的副作用和病毒突变而失去了疗效。本研究旨在确定并优化从新分离的蓝藻阿氏蓝藻Acaryochloris Al-Azhar MNE ON864448.1生产胞外多糖（EPS）的条件，同时探索其抗病毒活性。通过DEAE-52纤维素柱对蓝藻EPS进行纯化，最终产率为83.75%。应用不同的分析仪器对EPS进行鉴定，包括傅里叶变换红外（FT-IR）光谱、热重分析（TGA）和气相色谱-质谱联用（GC-MS）。Plackett-Burman设计表明，工作体积（X1）、EDTA（X2）、接种量（X3）、CaCl（X4）和NaCl（X5）是影响EPS产量的最重要变量。中心复合设计（CCD）显示，在1L容量瓶中工作体积为300mL、EDTA 0.002g/L、接种量7%、CaCl 0.046g/L和NaCl 20g/L的条件下，EPS产量最高（9.27mg/mL）。EPS在单纯疱疹病毒1型和2型（HSV-1、HSV-2）、腺病毒（ADV）和柯萨奇病毒（A16）感染的不同阶段均表现出强大的抗病毒活性。在HSV-1内化机制过程中记录到最高的半数抑制浓度（IC）（6.477μg/mL），而在柯萨奇病毒中和机制过程中记录到最低的IC（0.005669μg/mL）。
