来自蓝细菌（蓝藻）的抗艾滋病病毒硫脂。

AIDS-antiviral sulfolipids from cyanobacteria (blue-green algae).

作者信息

Gustafson K R, Cardellina J H, Fuller R W, Weislow O S, Kiser R F, Snader K M, Patterson G M, Boyd M R

机构信息

Division of Cancer Treatment, National Cancer Institute, Bethesda, MD.

出版信息

J Natl Cancer Inst. 1989 Aug 16;81(16):1254-8. doi: 10.1093/jnci/81.16.1254.

DOI:10.1093/jnci/81.16.1254

PMID:2502635

Abstract

A recently developed tetrazolium-based microculture assay was used to screen extracts of cultured cyanobacteria (blue-green algae) for inhibition of the cytopathic effects of the human immunodeficiency virus (HIV-1), which is implicated as a causative agent of AIDS. A number of extracts were found to be remarkably active against the AIDS virus. A new class of HIV-1-inhibitory compounds, the sulfonic acid-containing glycolipids, was discovered through the use of the microculture assay to guide the fractionation and purification process. The pure compounds were active against HIV-1 in cultured human lymphoblastoid CEM, MT-2, LDV-7, and C3-44 cell lines in the tetrazolium assay as well as in p24 viral protein and syncytium formation assays.

摘要

最近开发的一种基于四氮唑的微量培养测定法被用于筛选培养的蓝藻（蓝绿藻）提取物，以检测其对人类免疫缺陷病毒（HIV-1）细胞病变效应的抑制作用，HIV-1被认为是艾滋病的病原体。发现许多提取物对艾滋病病毒具有显著活性。通过使用微量培养测定法来指导分级分离和纯化过程，发现了一类新的HIV-1抑制化合物，即含磺酸的糖脂。在四氮唑测定法以及p24病毒蛋白和多核体形成测定中，这些纯化合物对培养的人淋巴母细胞样CEM、MT-2、LDV-7和C3-44细胞系中的HIV-1具有活性。