Cao Tong, Ho Kee-Hai, Teoh Swee-Hin
Faculty of Dentistry, National University of Singapore, Singapore.
Tissue Eng. 2003;9 Suppl 1:S103-12. doi: 10.1089/10763270360697012.
Tissue engineering offers an alternative method that can overcome some of the existing drawbacks of current articular defect repair methods because articular cartilage has a limited capacity to respond to injury. The solution may lie in the design of a three-dimensional load-bearing scaffold. Here we describe the tissue engineering of an osteochondral construct by coculturing osteogenic cells and chondrogenic cells on a three-dimensional load-bearing bioresorbable polymer scaffold. Porous polycaprolactone scaffolds were designed and fabricated via fused deposition modeling. Osteogenic cells were seeded and precultured in one-half of the partitioned scaffolds. Chondrogenic cells were later seeded into the other half. The cell-seeded scaffolds were cultured in a coculture medium. Both cell types proliferated, migrated, linked in their scaffold compartments, and integrated at the interface. Osteoblasts and chondrocytes produced different extracellular matrices in each scaffold compartment. Mineralized nodules deposited in the osteogenic cell seeded compartment. High osteocalcin was detected in precultured osteogenic cell supernatant and high alkaline phosphatase was detected in the coculture supernatant of osteochondral constructs. This study suggests that a tissue-engineered osteochondral construct with a three-dimensional polycaprolactone scaffold has the potential for osteochondral defect repair.
组织工程提供了一种替代方法，该方法可以克服当前关节缺损修复方法的一些现有缺点，因为关节软骨对损伤的反应能力有限。解决方案可能在于设计一种三维承重支架。在此，我们描述了通过在三维承重生物可吸收聚合物支架上共培养成骨细胞和软骨细胞来构建骨软骨结构的组织工程方法。通过熔融沉积建模设计并制造了多孔聚己内酯支架。将成骨细胞接种并预培养在分隔支架的一半中。随后将软骨细胞接种到另一半中。将接种细胞的支架在共培养基中培养。两种细胞类型均增殖、迁移，在其支架隔室中连接，并在界面处整合。成骨细胞和软骨细胞在每个支架隔室中产生不同的细胞外基质。矿化结节沉积在接种成骨细胞的隔室中。在预培养的成骨细胞上清液中检测到高骨钙素，在骨软骨构建体的共培养上清液中检测到高碱性磷酸酶。本研究表明，具有三维聚己内酯支架的组织工程骨软骨构建体具有修复骨软骨缺损的潜力。
