Washburn Christopher P, Bayliss Douglas A, Guyenet Patrice G
Department of Pharmacology, University of Virginia, Health Sciences Center, 1300 Jefferson Park Avenue P.O. Box 800735, Charlottesville, VA 22908-0735, USA.
Respir Physiol Neurobiol. 2003 Oct 16;138(1):19-35. doi: 10.1016/s1569-9048(03)00185-x.
The potassium channels TASK-1 and TASK-3 are neuronal 'leak' channels that are inhibited by extracellular acidification and numerous neurotransmitters. Here we tested whether in the rat TASK-1 and TASK-3 mRNAs are present in ventrolateral medulla neurons that regulate respiration or circulation (C1 adrenergic neurons). The mRNAs were identified by in situ hybridization. Respiratory neurons were identified by anatomical markers (neurokinin-1 receptors, NK1R, or somatostatin) or directly by juxtacellular labeling of bulbospinal neurons. C1 neurons were identified by the presence of tyrosine-hydroxylase. TASK-1 and TASK-3 transcripts were present in all hypoglossal and facial motor neurons, in most C1 cells (85-95%), in most small and large NK1R-ir neurons (>90%) of the ventral respiratory group (VRG) and in all the inspiratory-augmenting bulbospinal neurons of the rostral ventral respiratory group. In conclusion, TASK channels are expressed by respiratory cells with putative rhythmogenic function and by premotor and motor neurons. TASK channels presumably mediate excitatory effects of numerous transmitters on these neurons and may contribute to their pH-sensitivity.
钾通道TASK-1和TASK-3是神经元“渗漏”通道，可被细胞外酸化和多种神经递质抑制。在此，我们测试了大鼠中调节呼吸或循环的腹外侧延髓神经元（C1肾上腺素能神经元）中是否存在TASK-1和TASK-3 mRNA。通过原位杂交鉴定mRNA。通过解剖学标记物（神经激肽-1受体，NK1R，或生长抑素）或直接通过延髓脊髓神经元的近细胞标记来鉴定呼吸神经元。通过酪氨酸羟化酶的存在来鉴定C1神经元。TASK-1和TASK-3转录本存在于所有舌下和面部运动神经元、大多数C1细胞（85%-95%）、腹侧呼吸组（VRG）的大多数小和大的NK1R免疫反应性神经元（>90%）以及延髓头端腹侧呼吸组的所有吸气增强延髓脊髓神经元中。总之，TASK通道由具有假定节律生成功能的呼吸细胞以及运动前神经元和运动神经元表达。TASK通道可能介导多种递质对这些神经元的兴奋作用，并可能有助于它们的pH敏感性。
