Ueda Jun-Ya, Tezuka Yasuhiro, Banskota Arjun Hari, Tran Quan Le, Tran Qui Kim, Saiki Ikuo, Kadota Shigetoshi
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan.
Biol Pharm Bull. 2003 Oct;26(10):1431-5. doi: 10.1248/bpb.26.1431.
Sixteen cardenolides, two hemiterpenoids, two phenylpropanoids and a phenylethanoid isolated from the roots of Streptocaulon juventas (LOUR.) MERR. were examined for their antiproliferative activity toward three human-derived (HT-1080 fibrosarcoma, lung A549 adenocarcinoma, cervix HeLa adenocarcinoma) and three murine-derived (colon 26-L5 carcinoma, Lewis lung carcinoma, B16-BL6 melanoma) cell lines. The cardenolides selectively and strongly inhibited proliferation of the HT-1080 (IC(50) values, 0.054-1.6 microM) and A549 (IC(50), 0.016-0.65 microM) cell lines. The characteristic morphological changes and ladder-like DNA fragmentation in those cells treated with the cardenolides indicated the antiproliferative activity was due to the induction of apoptosis.
对从毛叶链珠藤（Streptocaulon juventas (LOUR.) MERR.）根部分离得到的16种强心苷、2种倍半萜、2种苯丙素和1种苯乙醇类化合物，检测了它们对三种人源细胞系（HT-1080纤维肉瘤细胞系、肺A549腺癌细胞系、宫颈HeLa腺癌细胞系）和三种鼠源细胞系（结肠26-L5癌细胞系、Lewis肺癌细胞系、B16-BL6黑色素瘤细胞系）的抗增殖活性。强心苷选择性且强烈地抑制HT-1080细胞系（半数抑制浓度（IC(50)）值为0.054 - 1.6微摩尔）和A549细胞系（IC(50)为0.016 - 0.65微摩尔）的增殖。用强心苷处理的那些细胞中特征性的形态变化和梯状DNA片段化表明抗增殖活性是由于诱导细胞凋亡所致。
