Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China.
Planta Med. 2013 Jan;79(2):157-62. doi: 10.1055/s-0032-1328021. Epub 2012 Dec 5.
Chemical investigation on the 75% ethanol extract of the roots of Streptocaulon juventas afforded two new cardiac glycosides, digitoxigenin 3-O-[O-β-D-glucopyranosyl-(1 → 4)-2-O-acetyl-β-D-digitalopyranoside] (1) and periplogenin 3-O-[O-β-D-glucopyranosyl-(1 → 4)-O-β-D-glucopyranosyl-(1 → 4)-β-D-cymaropyranoside] (2), and thirteen known cardenolides. Structures were elucidated by spectral methods. This is the first report of the isolation of compounds 3, 10, 14, and 15 from plants of the Streptocaulon genus, while 4, 11, and 12 are hitherto unreported from Streptocaulon juventas. All the compounds were in vitro evaluated for their cytotoxic activities against the A549 cell line, and seven effective cardiac glycosides were screened against the PC-9 cell line by WST assay, which also showed strong antiproliferation activities. Moreover, the characteristic morphological changes in PC-9 cells treated with cardenolides indicated cell inhibition due to apoptosis. These results revealed that these compounds possessed potential antitumor activities.
从筋骨草的 75%乙醇根提取物中进行的化学研究得到了两种新的强心苷，洋地黄毒苷 3-O-[O-β-D-吡喃葡萄糖基-(1 → 4)-2-O-乙酰基-β-D-吡喃葡萄糖苷]（1）和佩里普洛苷 3-O-[O-β-D-吡喃葡萄糖基-(1 → 4)-O-β-D-吡喃葡萄糖基-(1 → 4)-β-D-开环吡喃葡萄糖苷]（2），以及十三种已知的强心甾苷。结构通过光谱方法阐明。这是首次从筋骨草属植物中分离出化合物 3、10、14 和 15，而化合物 4、11 和 12 则是首次从筋骨草中分离出来的。所有化合物均在体外对 A549 细胞系进行了细胞毒性活性评估，并通过 WST 测定法对 7 种有效强心苷进行了抗 PC-9 细胞系的筛选，结果显示其具有很强的抗增殖活性。此外，用强心苷处理后的 PC-9 细胞的特征形态变化表明细胞因凋亡而受到抑制。这些结果表明这些化合物具有潜在的抗肿瘤活性。
