Panniiaĭnen V A, Krylov B V, Chalisova N I, Malevskaia I I
I. P. Pavlov Institute of Physiology RAS, St. Petersburg.
Tsitologiia. 2003;45(4):377-9.
Ouabain, a known inhibitor of Na+, K(+)-ATP, taken in a wide range of concentrations, was investigated in organotypic tissue culture of dorsal root ganglia cells of 10-11 day old chick embryos. Ouabain inhibited neurite growth in a dose-dependent manner. The Hill coefficient was defined as 1, and the Kp value was estimated as 1 x 10(-10) M. At inhibitor concentrations exceeding 1 x 10(-9) M, the growth of neurites was totally inhibited. It is assumed that Na+, K(+)-ATPase may play an important role in regulation of the process of neurite growth in sensory neurones.
