Ragupathi G, Prabhasankar P, Sekharan P C, Annapoorani K S, Damodaran C
Research and Development Division, Forensic Sciences Department, Madras, India.
Forensic Sci Int. 1992 Oct;56(2):127-36. doi: 10.1016/0379-0738(92)90170-2.
Cleistanthus collinus is a highly toxic plant frequently implicated in suicidal and homicidal poisoning cases referred to our laboratory. An enzyme-linked immunosorbent assay (ELISA) is reported in this paper for the quantitation of the active principles of C. collinus. This method is highly reproducible and sensitive to detect as low as 2 ng/ml of cleistanthin B, a toxic constituent of C. collinus. The ELISA can be successfully applied for the specific identification of C. collinus poisoning in clinical and forensic toxicology.
闭花木是一种剧毒植物，在送至我们实验室的自杀和他杀中毒案件中经常涉及。本文报道了一种用于定量闭花木活性成分的酶联免疫吸附测定法（ELISA）。该方法具有高度可重复性，对闭花木的有毒成分闭花木素B的检测灵敏度低至2 ng/ml。ELISA可成功应用于临床和法医毒理学中闭花木中毒的特异性鉴定。
