Ndiaye Mamadou, Chataigneau Thierry, Andriantsitohaina Ramaroson, Stoclet Jean-Claude, Schini-Kerth Valérie B
Pharmacologie et Physico-Chimie des Interactions Cellulaires et Moléculaires, UMR CNRS 7034, Faculté de Pharmacie, Université Louis Pasteur de Strasbourg, Strasbourg, France.
Biochem Biophys Res Commun. 2003 Oct 17;310(2):371-7. doi: 10.1016/j.bbrc.2003.09.028.
Moderate consumption of wine is associated with cardiovascular protection most likely by increasing the endothelial formation of nitric oxide (NO). The present study investigated whether red wine polyphenolic compounds (RWPCs) increase the formation of endothelium-derived hyperpolarizing factor (EDHF) in arteries and, if so, to characterize the underlying mechanism. Porcine coronary artery rings were suspended in organ chambers for measurement of changes in isometric tension and membrane potential in the presence of indomethacin and N(omega)-nitro-L-arginine. RWPCs caused pronounced endothelium-dependent relaxations and hyperpolarizations, which were reduced by the combination of charybdotoxin plus apamin (two inhibitors of EDHF-mediated responses). Both responses to RWPCs were also reduced by antioxidants, membrane permeant analogues of superoxide dismutase, and diphenylene iodonium, an inhibitor of flavin-dependent enzymes. RWPCs induced the formation of superoxide in cultured endothelial cells. These findings demonstrate that RWPCs cause EDHF-mediated relaxations of coronary arteries, which are critically dependent on a redox-sensitive mechanism involving a flavin-dependent enzyme.
适量饮用葡萄酒与心血管保护相关，最可能的原因是它能增加内皮细胞一氧化氮（NO）的生成。本研究调查了红酒多酚化合物（RWPCs）是否会增加动脉中内皮衍生超极化因子（EDHF）的生成，若会增加，则对其潜在机制进行表征。将猪冠状动脉环悬挂于器官浴槽中，在吲哚美辛和N（ω）-硝基-L-精氨酸存在的情况下测量等长张力和膜电位的变化。RWPCs引起明显的内皮依赖性舒张和超极化，这种作用被蝎毒素加蜂毒明肽（两种EDHF介导反应的抑制剂）的组合所减弱。对RWPCs的这两种反应也被抗氧化剂、超氧化物歧化酶的膜渗透性类似物以及黄素依赖性酶的抑制剂二苯碘鎓所减弱。RWPCs在培养的内皮细胞中诱导超氧化物的形成。这些发现表明，RWPCs引起冠状动脉的EDHF介导的舒张，这严重依赖于涉及黄素依赖性酶的氧化还原敏感机制。
