Horiuchi Takayuki, Giniger Edward, Aigaki Toshiro
Department of Biological Sciences, Tokyo Metropolitan University, Hachioji-shi, Tokyo 192-0397, Japan.
Genes Dev. 2003 Oct 15;17(20):2496-501. doi: 10.1101/gad.1137303. Epub 2003 Oct 1.
longitudinals lacking (lola) is a complex Drosophila gene encoding at least 20 protein isoforms,each bearing the same N-terminal constant region linked to a different C-terminal variable region. Different isoforms specify different aspects of axon growth and guidance. We show here that lola mRNAs are generated by alternative trans-splicing of exons sequentially encoded by the same DNA strand. Chromosomal pairing facilitates interallelic trans-splicing,allowing complementation between mutations in the constant and those in the variable exons. We demonstrate that at least one variable exon is transcribed from its own promoter,and trans-spliced to the constant exons transcribed separately.
缺失纵向结构（lola）是一个复杂的果蝇基因，编码至少20种蛋白质异构体，每种异构体都带有相同的N端恒定区，该恒定区与不同的C端可变区相连。不同的异构体决定轴突生长和导向的不同方面。我们在此表明，lola mRNA是由同一DNA链顺序编码的外显子的可变反式剪接产生的。染色体配对促进等位基因间的反式剪接，使恒定外显子和可变外显子中的突变之间能够互补。我们证明，至少有一个可变外显子从其自身的启动子转录，并反式剪接到单独转录的恒定外显子上。
