Millership S E, Want S V
Department of Infectious Diseases and Bacteriology, Hammersmith Hospital, London, United Kingdom.
J Clin Microbiol. 1992 Nov;30(11):2784-7. doi: 10.1128/jcm.30.11.2784-2787.1992.
A method of discriminating between strains of Mycobacterium tuberculosis by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins combined with a sensitive silver stain is described. Thirty-five isolates of M. tuberculosis and five isolates from other species of Mycobacterium were examined, including serial isolates from the same patients and isolates from a small cluster of hospital cases. Different species of Mycobacterium were clearly distinguished, and within the species M. tuberculosis, different fingerprints were obtained, allowing discrimination of some strains from different patients. The reproducibility and discrimination of the technique are discussed.
描述了一种通过对全细胞蛋白进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳并结合灵敏的银染法来鉴别结核分枝杆菌菌株的方法。对35株结核分枝杆菌分离株和5株来自其他分枝杆菌属的分离株进行了检测，包括同一患者的系列分离株和一小群医院病例的分离株。不同的分枝杆菌属被明显区分开，在结核分枝杆菌种内，获得了不同的指纹图谱，从而能够鉴别来自不同患者的一些菌株。讨论了该技术的可重复性和鉴别能力。
