Laboratory of Microbiology and Immunology, UR02-SP13, Universitary Hospital Farhat Hached, Sousse 4000, Tunisia.
Diagn Microbiol Infect Dis. 2011 Apr;69(4):396-9. doi: 10.1016/j.diagmicrobio.2010.11.009.
Identification of Mycobacterium tuberculosis complex (MTC) remains slow. Over the years, several new technologies have been proposed to accelerate and simplify the detection of MTC. In this context, we evaluated an immunochromatographic assay (ICA) (BIO-LINE SD Ag MPT64 TB) for rapid identification of MTC, based on detection of a specific MPT64 antigen of MTC. We have tested it on i) mycobacterial cultures: 210 MTC strains and 28 nontuberculous mycobacteria; ii) M. bovis bacille Calmette-Guérin strain SSI (Statens Serum Institut, Denmark); and iii) 22 microorganisms other than mycobacteria, isolated from cultures. We concluded that this kit has an excellent specificity (100%) and sensitivity (99%) from isolated cultures. The ICA (BIO-LINE SD Ag MPT64 TB) allows excellent MTC identification from clinical isolates. It is a rapid, simple, and inexpensive test, and has a definite contribution in the rapid laboratory diagnosis of tuberculosis.
结核分枝杆菌复合体（MTC）的鉴定仍然很慢。多年来，已经提出了几种新技术来加速和简化 MTC 的检测。在这种情况下，我们评估了一种基于 MTC 特异性 MPT64 抗原检测的免疫层析检测试剂盒（BIO-LINE SD Ag MPT64 TB），用于快速鉴定 MTC。我们对其进行了以下测试：i）分枝杆菌培养物：210 株 MTC 菌株和 28 株非结核分枝杆菌；ii）牛分枝杆菌卡介苗 SSI 株（丹麦 Statens Serum Institut）；和 iii）从培养物中分离的 22 种非分枝杆菌微生物。我们得出结论，该试剂盒从分离培养物中具有极好的特异性（100%）和敏感性（99%）。免疫层析检测试剂盒（BIO-LINE SD Ag MPT64 TB）可从临床分离物中出色地鉴定 MTC。它是一种快速、简单且廉价的检测方法，对结核病的快速实验室诊断有明确的贡献。
