Claas H C, Wagenvoort J H, Niesters H G, Tio T T, Van Rijsoort-Vos J H, Quint W G
Department of Molecular Biology, Diagnostic Centre SSDZ, Delft, The Netherlands.
J Clin Microbiol. 1991 Jan;29(1):42-5. doi: 10.1128/jcm.29.1.42-45.1991.
The diagnostic value of the polymerase chain reaction (PCR) for detection of Chlamydia trachomatis in comparison with that of the culture technique was established in a follow-up study of 32 patients (81 samples) who were treated for a C. trachomatis infection. The PCR was performed with two different sets of primers, a genus-specific primer set directed against the rRNA genes and a C. trachomatis-specific set directed against the common endogenous plasmid. After treatment with doxycycline, all patients became culture negative after 1 week. Results for the detection of C. trachomatis by the PCR were in complete agreement with the results by the culture method of detection, except for one culture-negative sample, which was found to be positive by the PCR. The results indicated that 1 week after treatment, no residual chlamydial DNA was found in the samples. Therefore, the PCR can be used for monitoring infections by chlamydiae.
在一项对32例接受沙眼衣原体感染治疗的患者（81份样本）的随访研究中，确定了聚合酶链反应（PCR）与培养技术相比在检测沙眼衣原体方面的诊断价值。PCR使用两组不同的引物进行，一组是针对rRNA基因的属特异性引物，另一组是针对常见内源质粒的沙眼衣原体特异性引物。用强力霉素治疗后，所有患者在1周后培养结果均为阴性。除一份培养阴性样本经PCR检测为阳性外，PCR检测沙眼衣原体的结果与培养检测方法的结果完全一致。结果表明，治疗1周后，样本中未发现残留的衣原体DNA。因此，PCR可用于监测衣原体感染。
