Misiewicz Irena, Skupinska Katarzyna, Kasprzycka-Guttman Teresa
Confocal Microscopy Laboratory, National Institute of Public Health, 00-725 Warsaw, Chelmska 30/34, Poland.
Oncol Rep. 2003 Nov-Dec;10(6):2045-50.
Flow cytometry and laser-scanning confocal microscopy were used to study the effect of sulforaphane (SFN) and 2-oxohexyl isothiocyanate on the growth and viability of mouse leukemia L-1210 and human melanoma ME-18 cells during their exponential growth. Sulforaphane belongs to a group of compounds known as isothiocyanates. Isothiocyanates mainly occur in Cruciferous family. In particular, they occur in many vegetables such as broccoli and their sprouts. SFN and 2-oxohexyl isothiocyanate are potent inducers of detoxication phase 2 enzymes in mouse tissues and murine hepatoma cells in culture. Sulforaphane was shown to induce cell growth arrest in a dose dependent manner, followed by cell death. Sulforaphane induced the cell death via an apoptotic process. Two markers of apoptosis were investigated: phosphatidylserine externalization, which occurs in the early stages of apoptosis, and DNA strand breaks. Our results strongly suggest of chemopreventive activity toward cancer by the induction of apoptosis by SFN and 2-oxohexyl isothiocyanate.
采用流式细胞术和激光扫描共聚焦显微镜，研究了萝卜硫素（SFN）和2-氧代己基异硫氰酸酯在小鼠白血病L-1210细胞和人黑色素瘤ME-18细胞指数生长期对其生长和活力的影响。萝卜硫素属于异硫氰酸酯类化合物。异硫氰酸酯主要存在于十字花科植物中。特别是，它们存在于许多蔬菜中，如西兰花及其芽苗菜。SFN和2-氧代己基异硫氰酸酯是小鼠组织和培养的小鼠肝癌细胞中解毒第二阶段酶的有效诱导剂。结果表明，萝卜硫素以剂量依赖的方式诱导细胞生长停滞，随后导致细胞死亡。萝卜硫素通过凋亡过程诱导细胞死亡。研究了凋亡的两个标志物：凋亡早期发生的磷脂酰丝氨酸外化和DNA链断裂。我们的结果有力地表明，SFN和2-氧代己基异硫氰酸酯通过诱导凋亡对癌症具有化学预防活性。
