Department of Drug Biotechnology and Bioinformatics, National Medicines Institute, 30/34 Chełmska St., 00-725 Warszawa, Poland.
Molecules. 2021 May 19;26(10):3019. doi: 10.3390/molecules26103019.
Combination therapy is based on the beneficial effects of pharmacodynamic interaction (synergistic or additive) between combined drugs or substances. A considerable group of candidates for combined treatments are natural compounds (e.g., isothiocyanates) and their analogs, which are tested in combination with anticancer drugs. We tested the anticancer effect of the combined treatment of isothiocyanate 2-oxohexyl isothiocyanate and 5-fluorouracil in colon and prostate cancer cell lines. The type of interaction was described using the Chou-Talalay method. The cytostatic and cytotoxic activities of the most promising combined treatments were investigated. In conclusion, we showed that combined treatment with 5-fluorouracil and 2-oxohexyl isothiocyanate acted synergistically in colon cancer. This activity is dependent on the cytostatic properties of the tested compounds and leads to the intensification of their individual cytotoxic activity. The apoptotic process is considered to be the main mechanism of cytotoxicity in this combined treatment.
联合治疗基于联合药物或物质之间药效学相互作用（协同或相加）的有益效果。相当一部分联合治疗候选者是天然化合物（例如异硫氰酸酯）及其类似物，它们与抗癌药物联合进行测试。我们测试了异硫氰酸酯 2-氧代己基异硫氰酸酯和 5-氟尿嘧啶联合治疗结肠和前列腺癌细胞系的抗癌效果。使用 Chou-Talalay 方法描述了相互作用的类型。研究了最有前途的联合治疗的细胞抑制和细胞毒性活性。总之，我们表明，5-氟尿嘧啶和 2-氧代己基异硫氰酸酯的联合治疗在结肠癌中表现出协同作用。这种活性取决于测试化合物的细胞抑制特性，并导致其单独细胞毒性活性的增强。细胞凋亡过程被认为是这种联合治疗中细胞毒性的主要机制。
