[染料木黄酮对体外培养成骨细胞增殖与分化的影响]
[Effects of genistein on proliferation and differentiation of cultured osteoblasts in vitro].
作者信息
Wang Jianhua, Kong Dejuan, Li En, Tong Xiaoxu, Chen Yongchun
机构信息
Institute of Combined Traditional Chinese and Western Medicine, Hebei Medical University, Shijiazhuang 050017.
出版信息
Zhong Yao Cai. 2002 Nov;25(11):803-5.
OBJECTIVE
To study the effects of genistein on proliferation and differentiation of cultured osteoblasts in vitro.
METHODS
MTT and PNPP were used separately to observe the proliferation and the activity of ALP of osteoblasts cultured in vitro with different concentration genistein for different incubation periods. Protein was measured by Lowry method.
RESULTS
It was found that genistein had the effects on promoting proliferation of osteoblasts cultured for 24 h and 48 h at concentration ranging from 1 x 10(-9) mol/L to 1 x 0(-5) mol/L. The activity of ALP was stimulated by genistein at concentration ranging from 1 x 10(-7) mol/L to 1 x 10(-5) mol/L after 72 h incubation.
CONCLUSION
Genistein has the effects on stimulating the proliferation and differentiation of cultured osteoblasts in vitro.
目的
研究染料木黄酮对体外培养成骨细胞增殖和分化的影响。
方法
分别采用MTT法和PNPP法，观察不同浓度染料木黄酮在不同培养时间下对体外培养成骨细胞增殖及碱性磷酸酶（ALP）活性的影响。采用Lowry法测定蛋白质含量。
结果
发现染料木黄酮在浓度为1×10⁻⁹mol/L至1×10⁻⁵mol/L范围内，对培养24小时和48小时的成骨细胞增殖有促进作用。培养72小时后，染料木黄酮在浓度为1×10⁻⁷mol/L至1×10⁻⁵mol/L范围内可刺激ALP活性。
结论
染料木黄酮具有刺激体外培养成骨细胞增殖和分化的作用。
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