抗生物素蛋白对生物素化的N-磺酰基-吖啶鎓-9-甲酰胺的区域依赖性发光猝灭

Regiodependent luminescence quenching of biotinylated N-sulfonyl-acridinium-9-carboxamides by avidin.

作者信息

Adamczyk Maciej, Mattingly Phillip G, Moore Jeffrey A, Pan You

机构信息

Department of Chemistry (D09MD), Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, AP20, Abbott Park, Illinois 60064-6016, USA.

出版信息

Org Lett. 2003 Oct 16;5(21):3779-82. doi: 10.1021/ol035323p.

DOI:10.1021/ol035323p

PMID:14535708

Abstract

[reaction: see text] Biotin was conjugated to chemiluminescent N-sulfonylacridinium-9-carboxamides at the N-10 or 9-position carboxamide. Upon binding to avidin, the light output of the N-10 derivative (8) was quenched up to 92% upon triggering with basic peroxide, while the 9-position carboxamide conjugate (9) was quenched only 33%. The utility of this effect was demonstrated in a model homogeneous chemiluminescence assay.

摘要

[反应：见正文] 生物素在N-10或9位羧酰胺处与化学发光的N-磺酰基吖啶鎓-9-羧酰胺缀合。与抗生物素蛋白结合后，用碱性过氧化物触发时，N-10衍生物（8）的发光输出淬灭高达92%，而9位羧酰胺缀合物（9）仅淬灭33%。这种效应的实用性在一个模型均相化学发光分析中得到了证明。