叶酸结合蛋白对蝶酸-N-磺酰基-吖啶鎓-9-甲酰胺缀合物的化学发光猝灭作用
Chemiluminescence quenching of pteroic acid-N-sulfonyl-acridinium-9-carboxamide conjugates by folate binding protein.
作者信息
Adamczyk Maciej, Fino James R, Mattingly Phillip G, Moore Jeffrey A, Pan You
机构信息
Department of Chemistry (D09MD), Abbott Laboratories, 100 Abbott Park Road, AP20, Abbott Park, IL 60064-6016, USA.
出版信息
Bioorg Med Chem Lett. 2004 May 3;14(9):2313-7. doi: 10.1016/j.bmcl.2004.01.105.
N(10)-Trifluoroacetylpteroic acid was conjugated to chemiluminescent N-sulfonylacridinium-9-carboxamide labels at the N(10) or 9-position carboxamide. Upon binding to folate binding protein the light output of the N(10) derivative (9) was quenched up to 62% upon triggering with basic peroxide, while the 9-position carboxamide conjugate (7) was quenched only 12%. The utility of this effect was demonstrated in a model homogeneous chemiluminescent assay for folic acid.
N(10)-三氟乙酰蝶酸在N(10)或9位羧酰胺处与化学发光的N-磺酰吖啶-9-羧酰胺标记物共轭。与叶酸结合蛋白结合后，用碱性过氧化物触发时，N(10)衍生物(9)的光输出淬灭高达62%，而9位羧酰胺共轭物(7)仅淬灭12%。这种效应的实用性在叶酸的模型均相化学发光测定中得到了证明。
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