Punab Margus, Lõivukene Krista, Kermes Kadri, Mändar Reet
Andrology Unit, United Laboratory of Tartu University Clinicum, University of Tartu, Tartu, Estonia.
Andrologia. 2003 Oct;35(5):271-8.
The aim of the study was to find out the correlation between white blood cell (WBC) counts in semen and quantitative composition of seminal microflora, and to establish the minimum WBC count associated with significant bacteriospermia. The research included 159 men with different WBC counts in their semen, 84 of them with chronic prostatitis/chronic pelvic pain syndrome. Semen samples were cultivated quantitatively for detecting anaerobic, microaerophilic and aerobic bacteria. Bryan-Leishman stained slides were used for detecting WBC in semen. Seminal fluid was colonized by eight different microorganisms, and the total count of microorganisms in semen ranged from 102 to 107 CFU ml-1. A high frequency of anaerobic microorganisms was found. A positive correlation was observed between the WBC count and the number of different microorganisms, and also between the WBC count and the total count of microorganisms in semen sample. The receiver operating characteristic curve analysis demonstrated that the WHO-defined WBC cut-off point (1 x 106 WBC ml-1) has very low sensitivity for discriminating between patients with and without significant bacteriospermia, as a more optimal sensitivity/specificity ratio appears at 0.2 x 106 WBC ml-1 of semen. The quantitative microbiological finding of semen in the patients of National Institute of Health (NIH) categories IIIa and IV was very similar, i.e. a high number of different microorganisms and a high total count of microorganisms. In the control group (without leucocytospermia and prostatitis symptoms) both parameters were significantly lower.
该研究的目的是找出精液中白细胞（WBC）计数与精液微生物群落定量组成之间的相关性，并确定与严重细菌性精液症相关的最低WBC计数。该研究纳入了159名精液中WBC计数不同的男性，其中84人患有慢性前列腺炎/慢性盆腔疼痛综合征。对精液样本进行定量培养以检测厌氧、微需氧和好氧细菌。使用Bryan-Leishman染色玻片检测精液中的WBC。精液中定植有8种不同的微生物，精液中微生物总数在102至107 CFU ml-1之间。发现厌氧微生物的频率较高。观察到WBC计数与不同微生物数量之间以及WBC计数与精液样本中微生物总数之间呈正相关。受试者工作特征曲线分析表明，世界卫生组织定义的WBC临界值（1×106 WBC ml-1）在区分有无严重细菌性精液症的患者时灵敏度非常低，因为在精液WBC计数为0.2×106 WBC ml-1时出现了更优的灵敏度/特异性比。美国国立卫生研究院（NIH）IIIa类和IV类患者精液的定量微生物学结果非常相似，即不同微生物数量多且微生物总数高。在对照组（无白细胞精子症和前列腺炎症状）中，这两个参数均显著较低。
