Zuker A, Tzfira T, Vainstein A
The Kennedy-Leigh Centre for Horticultural Research and The Otto Warburg Center for Biotechnology in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
Biotechnol Adv. 1998 Jan;16(1):33-79. doi: 10.1016/s0734-9750(97)00063-3.
The application of modern biotechnological approaches to cut flowers has clearly become instrumental and rewarding for the floriculture industry. In recent years, several gene-transfer procedures have been developed for some of the major commercial cut flowers. Using Agrobactrium or microprojectile bombardment, several basic protocols are now available. However, despite the great progress and interest in gene transfer to these crops, their transformation is routine in only a limited number of laboratories, and its application is still considered to be an "art form". This review summarizes the reported gene-transfer procedures for the main cut-flower crops, with an emphasis on the unique factors of each method and the recent progress in introducing new traits of horticultural interest into these species.
现代生物技术方法在切花中的应用显然已对花卉产业起到了重要作用并带来了收益。近年来，已针对一些主要的商业切花开发了多种基因转移程序。利用农杆菌或微粒轰击技术，现已具备几种基本方案。然而，尽管在这些作物的基因转移方面取得了巨大进展并引发了人们的兴趣，但它们的转化仅在有限数量的实验室中是常规操作，其应用仍被视为一种“艺术形式”。本综述总结了已报道的主要切花作物的基因转移程序，重点介绍了每种方法的独特因素以及将具有园艺价值的新性状引入这些物种方面的最新进展。
