Gribanov G A, Golovko M Iu, Borinskiĭ Iu N, Diachkova L Ia
Tver State University.
Biomed Khim. 2003 May-Jun;49(3):267-72.
The influence of exogenous ions Ca2+ on the autholytic alterations of lipids in the brain grey matter of rats was investigated during incubation in a sterile physiological solution in vitro. The level of phospholipids decreased with parallel accumulation of diacylglycerols and free fatty acids. The contents of cholesterol and its esters varied in reciprocal oscillatory mode with prevalence of the disintegration of cholesterol esters started after 4 hrs of autholysis and further. The most probable degradation reactions of phospholipids and also cholesterol esters were the hydrolytic reactions. Transacylation reactions of between phospholipids, cholesterol and its esters were also possible. The presence of exogenous Ca2+ ions in the incubation medium promoted decrease of the total lipid grey matter. This effect was noted 10 min after onset of autolysis and continued up to 24 h. The reduction of lipid concentration occurred due to the decrease of phospholipid level and to a lesser extent--to the cholesterol level.
在体外无菌生理溶液中孵育期间，研究了外源离子Ca2+对大鼠脑灰质脂质自溶变化的影响。磷脂水平下降，同时二酰甘油和游离脂肪酸平行积累。胆固醇及其酯的含量呈反向振荡模式变化，自溶4小时及之后开始，胆固醇酯分解占优势。磷脂以及胆固醇酯最可能的降解反应是水解反应。磷脂、胆固醇及其酯之间的转酰基反应也是可能的。孵育培养基中外源Ca2+离子的存在促进了脑灰质总脂质的减少。自溶开始10分钟后即观察到这种效应，并持续至24小时。脂质浓度的降低是由于磷脂水平的下降，以及程度较小的胆固醇水平的下降。
