Gribanov G A, Il'iashenko D V
Vopr Med Khim. 1993 Mar-Apr;39(2):43-5.
A decrease in relative content of phospholipids and cholesterol simultaneously with increase in cholesterol esters and free fatty acids were detected in tissues of rat brain gray and white matters during autolysis at 37 degrees within 6-7 min, 1, 4 and 24 hrs; the most distinct alterations were observed in lipids of the gray matter especially at early (6-7 min) and late (24 hrs) stages of autolysis. In the gray matter, relative content of all the lipid fractions studied was restored to initial level within 4 hrs of incubation. In the white matter, during autolysis the content of cholesterol varied, the content of phospholipids was only slightly increased, while the level of free fatty acids was increased only at 24 hrs of incubation with synchronous decrease in content of triacylglycerols, cholesterol and, partially, phospholipids. These experimental data are of importance in resuscitation. Both common and dissimilar mechanisms of these lipid alterations are discussed. Not only the complex of hydrolase reactions but also that of transacylase and other reactions, involved in the degradation and biotransformation of brain lipids in autolysis, were noted.
在37℃下自溶6 - 7分钟、1小时、4小时和24小时期间，在大鼠脑灰质和白质组织中检测到磷脂和胆固醇的相对含量下降，同时胆固醇酯和游离脂肪酸增加；在灰质脂质中观察到最明显的变化，尤其是在自溶的早期（6 - 7分钟）和晚期（24小时）阶段。在灰质中，所有研究的脂质组分的相对含量在孵育4小时内恢复到初始水平。在白质中，自溶期间胆固醇含量变化，磷脂含量仅略有增加，而游离脂肪酸水平仅在孵育24小时时增加，同时三酰甘油、胆固醇以及部分磷脂的含量同步下降。这些实验数据在复苏方面具有重要意义。讨论了这些脂质变化的共同和不同机制。不仅注意到了水解酶反应的复合体，还注意到了参与自溶过程中脑脂质降解和生物转化的转酰基酶反应及其他反应的复合体。
