大肠杆菌中的动态结构：MinE环和MinD极性区的自发形成
Dynamic structures in Escherichia coli: spontaneous formation of MinE rings and MinD polar zones.
作者信息
Huang Kerwyn Casey, Meir Yigal, Wingreen Ned S
机构信息
Department of Physics, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 12-111, Cambridge, MA 02139, USA.
出版信息
Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12724-8. doi: 10.1073/pnas.2135445100. Epub 2003 Oct 20.
Abstract
In Escherichia coli, division site selection is regulated in part by the Min-protein system. Oscillations of the Min proteins from pole to pole every approximately 40 sec have been revealed by in vivo studies of GFP fusions. The dynamic oscillatory structures produced by the Min proteins, including a ring of MinE protein, compact polar zones of MinD, and zebra-striped oscillations in filamentous cells, remain unexplained. We show that the Min oscillations, including mutant phenotypes, can be accounted for by in vitro-observed interactions involving MinD and MinE, with a crucial role played by the rate of nucleotide exchange. Recent discoveries suggest that protein oscillations may play a general role in proper chromosome and plasmid partitioning.
摘要
在大肠杆菌中，细胞分裂位点的选择部分受Min蛋白系统调控。通过对绿色荧光蛋白（GFP）融合蛋白进行体内研究，发现Min蛋白每隔约40秒在两极之间振荡一次。Min蛋白产生的动态振荡结构，包括MinE蛋白环、MinD紧密的极性区域以及丝状细胞中的斑马条纹状振荡，其原因仍不清楚。我们发现，包括突变体表型在内的Min振荡，可以通过体外观察到的涉及MinD和MinE的相互作用来解释，其中核苷酸交换速率起着关键作用。最近的发现表明，蛋白质振荡可能在染色体和质粒的正确分配中普遍发挥作用。
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Recruitment of MinC, an inhibitor of Z-ring formation, to the membrane in Escherichia coli: role of MinD and MinE.MinC（一种Z环形成抑制剂）在大肠杆菌中向细胞膜的募集：MinD和MinE的作用。
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The Min system is not required for precise placement of the midcell Z ring in Bacillus subtilis.在枯草芽孢杆菌中，Min系统并非精确放置细胞中部Z环所必需的。
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