1918年之前的禽流感血凝素（HA）和核蛋白（NP）序列与后续禽流感毒株的关系。

Relationship of pre-1918 avian influenza HA and NP sequences to subsequent avian influenza strains.

作者信息

Reid A H, Fanning T G, Slemons R D, Janczewski T A, Dean J, Taubenberger J K

机构信息

Division of Molecular Pathology, Department of Cellular Pathology and Genetics, Armed Forces Institute of Pathology, 1413 Research Boulevard, Building 101, Room 1057D, Rockville, MD 20850-3125, USA.

出版信息

Avian Dis. 2003;47(3 Suppl):921-5. doi: 10.1637/0005-2086-47.s3.921.

DOI:10.1637/0005-2086-47.s3.921

PMID:14575087

Abstract

Wild waterfowl that were captured between 1915 and 1919 and preserved in 70% ethyl alcohol were tested for influenza A virus RNA. Most of the HA1 domain of the hemagglutinin (HA) gene segment was sequenced from one bird, captured in 1917, that was infected with a virus of the same HA subtype as the 1918 human pandemic virus. The 1917 HA sequence is closely related to modern avian HA sequences, suggesting little drift in avian sequences in 80 years and that the 1918 pandemic virus probably did not acquire its hemagglutinin directly from a bird. A 151-bp fragment of the nucleoprotein gene segment was sequenced from two pre-1918 birds and compared to avian and mammalian influenza strains. The 1917 avian NP sequences are also closely related to modern avian sequences and distinct from the mammalian clade in which the 1918 NP sequence is found.

摘要

对1915年至1919年间捕获并保存在70%乙醇中的野生水禽进行了甲型流感病毒RNA检测。从1917年捕获的一只感染了与1918年人类大流行病毒相同HA亚型病毒的鸟类中，对血凝素（HA）基因片段的大部分HA1结构域进行了测序。1917年的HA序列与现代禽类HA序列密切相关，这表明80年来禽类序列几乎没有漂移，并且1918年大流行病毒可能并非直接从鸟类获得其血凝素。从两只1918年前的鸟类中对核蛋白基因片段的一个151bp片段进行了测序，并与禽类和哺乳动物流感毒株进行了比较。1917年的禽类NP序列也与现代禽类序列密切相关，并且与发现1918年NP序列的哺乳动物进化枝不同。