Solé Montserrat, Raldua Demetrio, Barceló Damià, Porte Cinta
Environmental Chemistry Department, IIQAB-CSIC, Jordi Girona, 18-26, 08034, Barcelona, Spain.
Ecotoxicol Environ Saf. 2003 Nov;56(3):373-80. doi: 10.1016/s0147-6513(02)00143-4.
In the present study, the estrogenic response in the reproductive cycle of female carp, Cyprinus carpio, in relation to a sewage treatment works (STWs) was measured as: alterations in plasmatic and hepatic vitellogenin (VTG) content, plasma levels of testosterone (T) and estradiol (E2), and effects on key enzymes of phases I and II biotransformation, ethoxyresorufin O-deethylase (EROD) and glutathione S-transferase (GST). Low plasma VTG content in females collected 4 km downstream of an effluent discharge suggested possible estrogenic disturbances; however, hepatic VTG content and sex hormone levels did not mirror this. In fact, E2 and T largely fluctuated among fish, with individual variations even greater than among sampling stations or periods. This suggests that at moderately polluted sites, biological variations of female carp and genetic particularities are prevalent. Hepatic biotransformation enzymes, such as EROD, increased with increasing water temperature while, in contrast, GST was maximal at the lowest water temperature.
在本研究中，测定了雌性鲤鱼（Cyprinus carpio）生殖周期中与污水处理厂（STWs）相关的雌激素反应，指标如下：血浆和肝脏中卵黄蛋白原（VTG）含量的变化、睾酮（T）和雌二醇（E2）的血浆水平，以及对I相和II相生物转化关键酶乙氧基异吩恶唑酮O-脱乙基酶（EROD）和谷胱甘肽S-转移酶（GST）的影响。在废水排放口下游4公里处采集的雌性鲤鱼血浆VTG含量较低表明可能存在雌激素干扰；然而，肝脏VTG含量和性激素水平并未反映出这一点。事实上，E2和T在鱼之间波动很大，个体差异甚至大于采样站或采样时期之间的差异。这表明在中度污染的地点，雌性鲤鱼的生物学变异和遗传特性很普遍。肝脏生物转化酶，如EROD，随着水温升高而增加，而相比之下，GST在最低水温时达到最大值。
