Miyawaki Atsushi, Nagai Takeharu, Mizuno Hideaki
Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan.
Curr Opin Chem Biol. 2003 Oct;7(5):557-62. doi: 10.1016/s1367-5931(03)00097-8.
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and GFP-like proteins from Anthozoa species contain light-absorbing chromophores within their protein sequences. Recent studies have made progress in obtaining bright variants of these proteins that develop chromophores quickly and efficiently, as well as novel fluorescent proteins that photoconvert (i.e. change color upon illumination at specific wavelengths). Further molecular characterization of the structure and maturation of these proteins is in progress, aimed at providing information for rational design of variants with desired fluorescence properties.
来自维多利亚多管水母的绿色荧光蛋白（GFP）以及来自珊瑚纲物种的类GFP蛋白在其蛋白质序列中含有吸光发色团。最近的研究在获得能快速高效形成发色团的这些蛋白的明亮变体，以及光转换（即在特定波长光照下改变颜色）的新型荧光蛋白方面取得了进展。对这些蛋白的结构和成熟过程的进一步分子表征正在进行中，目的是为合理设计具有所需荧光特性的变体提供信息。
