Cyster L A, Parker K G, Parker T L, Grant D M
Bioengineering Group, School of MMMEM, University of Nottingham,Nottingham NG7 2RD, UK.
Biomaterials. 2004 Jan;25(1):97-107. doi: 10.1016/s0142-9612(03)00480-0.
The cell-substrate interaction of primary hippocampal neurones with thin films of TiN was studied in vitro. TiN films of different surface chemistries and topographies were deposited by pulsed DC reactive magnetron sputtering and closed field unbalanced magnetron sputter ion plating by Teer Coatings Ltd., Hartlebury, UK to result in TiN films with similar surface chemistries but different topographical features. TiN films were characterised using X-ray diffraction, X-ray photoelectron spectroscopy and atomic force microscopy. The neuron-substrate interaction was examined using environmental scanning electron microscopy (FEG-ESEM) for morphological information. Bromodeoxyuridine and TUNEL assays were used to identify proliferating neurones as well as apoptotic neurones. Fluorescent staining for MAP-2 was used to label neuronal network formation. Primary hippocampal neurones were found to attach and spread to all of the TiN film chemistries and topographies investigated. Neuronal network morphology appeared to be more preferential on the nitrogen rich TiN films and also with reduced nanotopographical features.
在体外研究了原代海马神经元与氮化钛薄膜的细胞-基质相互作用。英国哈特尔伯里的蒂尔涂层有限公司通过脉冲直流反应磁控溅射和闭合场非平衡磁控溅射离子镀沉积了具有不同表面化学性质和形貌的氮化钛薄膜，从而得到具有相似表面化学性质但不同形貌特征的氮化钛薄膜。使用X射线衍射、X射线光电子能谱和原子力显微镜对氮化钛薄膜进行了表征。使用环境扫描电子显微镜（场发射枪环境扫描电子显微镜）获取形态学信息，以研究神经元-基质相互作用。使用溴脱氧尿苷和TUNEL检测法来鉴定增殖神经元以及凋亡神经元。使用针对微管相关蛋白2（MAP-2）的荧光染色来标记神经网络的形成。研究发现，原代海马神经元能够附着并铺展在所研究的所有氮化钛薄膜的化学性质和形貌上。神经网络形态似乎在富氮的氮化钛薄膜上更具优势，并且纳米形貌特征减少时也是如此。
