Cyster L A, Parker K G, Parker T L, Grant D M
Biomaterials Group, School of MMMEM, University of Nottingham, Nottingham, NG7 2RD, United Kingdom.
J Biomed Mater Res A. 2003 Oct 1;67(1):138-47. doi: 10.1002/jbm.a.10087.
The cell-material interaction of 3T3-L1 fibroblasts with TiN films was studied in vitro. TiN films were deposited onto glass substrates to thicknesses of 0.2 and 1.0 microm by pulsed dc reactive magnetron sputtering. For comparison TiN films were deposited by closed field unbalanced magnetron sputter ion plating by Teer Coatings Ltd. (Hartlebury, UK) to result in TiN films with similar surface chemistries but having increased topographical features. TiN films were characterized using X-ray diffraction, X-ray photoelectron spectroscopy, and atomic force microscopy. The cell-material interaction was examined morphologically by monitoring fibroblast attachment and growth and comparing to a control substrate. At early time points increased numbers of 3T3-L1 fibroblasts were found to preferentially attach to TiN films with an increase in the percentage of surface interstitial nitrogen and also with decreased topographical features. At later time points the presence of nanotopography appeared to play a greater role than the effects of surface chemistry and resulted in increased numbers of attached 3T3-L1 fibroblasts. The results show that by changing the deposition route and parameters to produce TiN films, the resultant films can be used to investigate the cellular response to surfaces of differing chemistry and topography.
在体外研究了3T3-L1成纤维细胞与氮化钛（TiN）薄膜的细胞-材料相互作用。通过脉冲直流反应磁控溅射将TiN薄膜沉积在玻璃基板上，厚度分别为0.2微米和1.0微米。作为比较，由英国哈特尔伯里的蒂尔涂层有限公司通过闭合场不平衡磁控溅射离子镀沉积TiN薄膜，以得到具有相似表面化学性质但具有增强形貌特征的TiN薄膜。使用X射线衍射、X射线光电子能谱和原子力显微镜对TiN薄膜进行表征。通过监测成纤维细胞的附着和生长并与对照基板进行比较，从形态学上检查细胞-材料相互作用。在早期时间点，发现3T3-L1成纤维细胞数量增加，优先附着于表面间隙氮百分比增加且形貌特征减少的TiN薄膜。在后期时间点，纳米形貌的存在似乎比表面化学作用发挥更大作用，并导致附着的3T3-L1成纤维细胞数量增加。结果表明，通过改变沉积途径和参数来制备TiN薄膜，所得薄膜可用于研究细胞对不同化学性质和形貌表面的反应。
